Invitrogen™

SuperScript™ III Reverse Transcriptase

Catalog number:  18080093

Related applications:

Reverse Transcription

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Description

Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) that was created by introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability. SuperScript III RT offers higher cDNA yields, improved cDNA lengths, improved efficiency on GC-rich target RNAs, and overall better performance than wild-type MMLV and MMLV RNase H-minus enzymes.

SuperScript RTs are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date.

Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase, features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Enzyme Function: RNA dependent DNA Polymerase
Optimal Reaction Temperature: 50° C
Product Line: SuperScript™
Quantity: 2000 units
Reverse Transcriptase: SuperScript™ III
Ribonuclease H Activity: Reduced
Sensitivity: Medium
Shipping Condition: Dry Ice
Template: ssRNA
Concentration: 200 U⁄µl
Detection Method: Primer-Probe
Form: Frozen
Format: Tube(s)
GC-Rich PCR Performance: High
No. of Reactions: 10 Reactions
No. of Tests: 10 Reactions
PCR Method: RT-PCR
Product Type Specs: Reverse Transcriptase
Size (Final Product): 12.3 kb or less
Sufficient For: 10 Reactions
Volume: 200 μL

Contents & storage

SuperScript™ III Reverse Transcriptase (2,000 units total, at 200U/µL) is supplied with a vial (1 mL) of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial (500 µL) of 100 mM DTT. Store at -20°C.