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Invitrogen™

SuperScript™ III First-Strand Synthesis SuperMix

Catalog number: 18080400
Invitrogen™

SuperScript™ III First-Strand Synthesis SuperMix

Catalog number: 18080400
Catalog Number
18080400
also known as 18080-400
Unit Size
50 rxns
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
18080400
also known as 18080-400
50 rxns
-
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The SuperScript III First-Strand Synthesis SuperMix is an optimized formulation for first-strand cDNA synthesis from purified poly(A)+ or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system. The amount of starting material can vary from 0.1 pg to 5 μg of total RNA.

This kit includes SuperScript III/RNaseOUT enzyme mix, 2X First-Strand reaction mix, and an annealing buffer. SuperScript III Reverse Transcriptase is a version of MMLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme can be used to synthesize cDNA at a temperature range of 45–55°C, providing increased specificity, higher yields of cDNA, and more full-length product than many previous reverse transcriptases.

Using SuperScript III First-Strand Synthesis SuperMix
SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA and can be used to synthesize cDNA from total RNA. RNaseOUT Recombinant RNase Inhibitor is included in the enzyme mix to safeguard against degradation of target RNA due to ribonuclease contamination. The 2X First-Strand reaction mix includes 10 mM MgCl2 and 1 mM of each dNTP in a buffer formulation that has been optimized for first-strand synthesis of cDNA. The annealing buffer is used in the initial template-primer annealing step. Separate tubes of oligo(dT)20 and random hexamers are also provided. cDNA synthesis can be performed using either total RNA or poly(A)+-selected RNA primed with oligo(dT), random primers, or a gene-specific primer.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Kit
GC-Rich PCR Performance
High
Reaction Speed
50 min.
Technique
Reverse Transcription
Optimal Reaction Temperature
50°C
Primer
Random Primers, Oligo dT Primers
Reverse Transcriptase
SuperScript III
Shipping Condition
Dry Ice
For Use With (Application)
Real Time PCR (qPCR)
Final Product Type
First-Strand cDNA
No. of Reactions
50 Reactions
Reaction Format
Master Mix
Reagent Type
Reverse Transcription
Starting Material
RNA

Contents & Storage

• 2X Reaction Mix, 500 μL
• SuperScript™ III Enzyme Mix, 100 μL
• Oligo(dT)20, 50 μL total (50 μM)
• Random hexamers, 50 μL (50 ng/μL)
• Annealing buffer, 50 μL

Store at –20°C.

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