SuperScript® Choice System for cDNA Synthesis

Catalog number: 18090019

Invitrogen™  Related applications: cDNA Libraries & Library Construction

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The SuperScript® Choice System is designed for synthesis of double-stranded cDNA from a purified mRNA population. Advantages of the SuperScript® Choice system include:

• The advanced capabilities of SuperScript® II RT resulting in greater cDNA yields and increased full-length cDNAs (1,3)
• Choice of primers for first-strand synthesis (2)
• One-tube format improving cDNA yield (4,5)
• cDNA is size-fractionated using an efficient and convenient pre-packed column
• One reaction converts up to 5 µg of mRNA into size-fractionated, EcoR I-adapted cDNA, ready for ligation into any EcoR I-digested vector

Performance and Quality Testing: Yields of the first- and secondstrand cDNA synthesis reactions and percentage of full-length double-stranded cDNA are assessed using the control RNA.
For Research Use Only. Not for use in diagnostic procedures.



Reverse Transcriptase: SuperScript® II
Downstream Application: cDNA Library Construction
Reaction Format: Separate Components
Sample Type (General): RNA
Product Size: 3 reactions
Final Product: cDNA (Second-Strand)
Optimal Reaction Temperature: 42° C

Contents & storage

The SuperScript® Choice System for cDNA Synthesis includes all components listed in the table. Store at -20°C, except for cDNA size fractionation columns which are stored at +4°C.


Manuals & protocols

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