Invitrogen™

Subcloning Efficiency™ DH5α Competent Cells

Catalog number:  18265017

Related applications:

Transformation

Back to top

Description

Subcloning Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that provide a transformation efficiency of > 1 x 106 cfu/µg plasmid DNA. Subcloning Efficiency DH5α Competent Cells are an economical solution for routine subcloning procedures or any application where the starting DNA is not limiting.

Features of Subcloning Efficiency DH5α Competent Cells:

• Designed for general, everyday use
• Contain genetic markers useful for general applications

Ideal for routine subcloning procedures
Subcloning Efficiency DH5α Competent Cells are recommended for routine subcloning of genes into plasmid vectors but are not suitable for the generation of cDNA libraries. These economical cells yield >1 x 106 transformants/µg control DNA per 50 µL reaction.

Flexible cloning capabilities
Subcloning Efficiency DH5α Competent Cells contain the following genetic markers resulting in these benefits:

lacZΔM15 for blue/white color screening of colonies on plates containing X-gal or Bluo-gal
recA1 ensures increased insert stability and prevents unwanted recombination
endA1 improves the yield and quality of plasmid DNA prepared from minipreps
• DH5α competent cells support replication of M13mp vectors but do not support plaque formation

Genotype: F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 λ-
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain: DH5α
Blue-White Screening: Yes
Cloning Methylated DNA: No
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Competent Cell Type: Chemically Competent
F' Episome (to make ssDNA): Lacks F' Episome
Format: Tube(s)
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Improves Plasmid Quality: Yes
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Line: DH5a,Subcloning Efficiency
Product Size: 500 µL
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: Yes
Shipping Condition: Dry Ice
Species: E. coli
T1 Phage - Resistant (tonA): No
Transformation Efficiency: > 1x10^6

Contents & storage

Contains:
• Subcloning Efficiency DH5α Competent Cells: 4 vials, 500 µl each (total of 2 ml)
• pUC19 DNA (100 pg/ul): 1 vial, 20 µl

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C.