Subcloning Efficiency™ DH5α™ Competent Cells

Subcloning Efficiency™ DH5α™ Competent Cells

Catalog number: 18265017

Invitrogen™  Related applications: Transformation

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Description

Subcloning Efficiency™ DH5α™ Competent Cells are a versatile strain of chemically competent cells that provide a transformation efficiency of > 1 x 106 cfu/µg plasmid DNA. Subcloning Efficiency™ DH5α™ Competent Cells are an economical solution for routine subcloning procedures or any application where the starting DNA is not limiting.

Features of Subcloning Efficiency™ DH5α™ Competent Cells:

• Designed for general, everyday use
• Contain genetic markers useful for general applications

Ideal for routine subcloning procedures
Subcloning Efficiency™ DH5α™ Competent Cells are recommended for routine subcloning of genes into plasmid vectors but are not suitable for the generation of cDNA libraries. These economical cells yield >1 x 106 transformants/µg control DNA per 50 µL reaction.

Flexible cloning capabilities
Subcloning Efficiency™ DH5α™ Competent Cells contain the following genetic markers resulting in these benefits:

• lacZΔM15 for blue/white color screening of colonies on plates containing X-gal or Bluo-gal
• recA1 ensures increased insert stability and prevents unwanted recombination
• endA1 improves the yield and quality of plasmid DNA prepared from minipreps
• DH5α™ competent cells support replication of M13mp vectors but do not support plaque formation

Genotype: F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 λ-
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Competent Cell Type: Chemically Competent
Species: E. coli
Format: Tube(s)
Transformation Efficiency: > 1x10^6
Bacterial or Yeast Strain: DH5α™
Product Size: 40 reactions
Blue-White Screening: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Reduces Recombination: Yes
Cloning Methylated DNA: No
Improves Plasmid Quality: Yes
Propagating ccdB Vectors: Not for ccdB vector propagation
F' Episome (to make ssDNA): Lacks F' Episome
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA): No
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Shipping Condition: Dry Ice

Contents & storage

Contains:
• Subcloning Efficiency™ DH5α™ Competent Cells: 4 vials, 500 µl each (total of 2 ml)
• pUC19 DNA (100 pg/ul): 1 vial, 20 µl

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C.

Documents

Manuals & protocols

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