Invitrogen™

Subcloning Efficiency™ DH5α Competent Cells

Catalog number: 18265017
Invitrogen™

Subcloning Efficiency™ DH5α Competent Cells

Catalog number: 18265017
Catalog Number
18265017
Unit Size
4 x 500 µL
Price (USD)
Price: 109.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
182650174 x 500 µL
Price: 109.00
Your Price:
-

Recurring order eligible

Save time by having your items shipped automatically.

Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations
Subcloning Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that provide a transformation efficiency of > 1 x 106 cfu/µg plasmid DNA. Subcloning Efficiency DH5α Competent Cells are an economical solution for routine subcloning procedures or any application where the starting DNA is not limiting.

Features of Subcloning Efficiency DH5α Competent Cells:

• Designed for general, everyday use
• Contain genetic markers useful for general applications

Ideal for routine subcloning procedures
Subcloning Efficiency DH5α Competent Cells are recommended for routine subcloning of genes into plasmid vectors but are not suitable for the generation of cDNA libraries. These economical cells yield >1 x 106 transformants/µg control DNA per 50 µL reaction.

Flexible cloning capabilities
Subcloning Efficiency DH5α Competent Cells contain the following genetic markers resulting in these benefits:

lacZΔM15 for blue/white color screening of colonies on plates containing X-gal or Bluo-gal
recA1 ensures increased insert stability and prevents unwanted recombination
endA1 improves the yield and quality of plasmid DNA prepared from minipreps
• DH5α competent cells support replication of M13mp vectors but do not support plaque formation

Genotype: F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 λ-
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Competent Cell
Contains F' Episome
Lacks F' Episome
Efficiency
Subcloning Efficiency (10^6-10^7 cfu/µg)
Cell Type
Chemically Competent
Improves Plasmid Quality
Yes
Cloning Methylated DNA
No
Transformation Efficiency Level
Subcloning Efficiency (10^6-10^7 cfu⁄µg)
Cloning Unstable DNA
Not Suitable for cloning unstable DNA
Bacterial or Yeast Strain
DH5α
Blue/White Screening
Yes
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
Propagating ccdB Vectors
Not for ccdB vector propagation
Reduces Recombination
Yes
Shipping Condition
Dry Ice
T1 Phage - Resistant (tonA)
No
Species
E. coli
Format
Tube
Product Line
DH5a, Subcloning Efficiency
For Use With (Application)
Cloning

Contents & Storage

Contains:
• Subcloning Efficiency DH5α Competent Cells: 4 vials, 500 µl each (total of 2 ml)
• pUC19 DNA (100 pg/ul): 1 vial, 20 µl

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C.