Subcloning Efficiency™ DH5α™ Competent Cells

Description

Subcloning Efficiency™ DH5α™ Competent Cells are a versatile strain of chemically competent cells that provide a transformation efficiency of > 1 x 10⁶ cfu/µg plasmid DNA. Subcloning Efficiency™ DH5α™ Competent Cells are an economical solution for routine subcloning procedures or any application where the starting DNA is not limiting.

Features of Subcloning Efficiency™ DH5α™ Competent Cells:

• Designed for general, everyday use
• Contain genetic markers useful for general applications

Ideal for routine subcloning procedures
Subcloning Efficiency™ DH5α™ Competent Cells are recommended for routine subcloning of genes into plasmid vectors but are not suitable for the generation of cDNA libraries. These economical cells yield >1 x 10⁶ transformants/µg control DNA per 50 µL reaction.

Flexible cloning capabilities
Subcloning Efficiency™ DH5α™ Competent Cells contain the following genetic markers resulting in these benefits:

• lacZΔM15 for blue/white color screening of colonies on plates containing X-gal or Bluo-gal
• recA1 ensures increased insert stability and prevents unwanted recombination
• endA1 improves the yield and quality of plasmid DNA prepared from minipreps
• DH5α™ competent cells support replication of M13mp vectors but do not support plaque formation

Genotype: F^- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(r_k^-, m_k⁺) phoA supE44 thi-1 gyrA96 relA1 λ^-

Specifications

Competent Cell Type:	Chemically Competent
Species:	E. coli
Format:	Tube(s)
Transformation Efficiency:	> 1x10^6
Bacterial or Yeast Strain:	DH5α™
Product Line:	DH5a™, Subcloning Efficiency™
Blue-White Screening:	Yes
Cloning Methylated DNA:	No
Cloning Unstable DNA:	Not Suitable for cloning unstable DNA
F' Episome (to make ssDNA):	Lacks F' Episome
High Throughput Compatibility:	Not High Throughput-Compatible (Manual)
Improves Plasmid Quality:	Yes
Preparing Unmethylated DNA:	Not Suitable for preparing unmethylated DNA
Product Size:	40 reactions
Propagating ccdB Vectors:	Not for ccdB vector propagation
Reduces Recombination:	Yes
T1 Phage - Resistant (tonA):	No
Shipping Condition:	Dry Ice

Contents & storage