Subcloning Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that provide a transformation efficiency of > 1 x 10⁶ cfu/µg plasmid DNA. Subcloning Efficiency DH5α Competent Cells are an economical solution for routine subcloning procedures or any application where the starting DNA is not limiting.

Features of Subcloning Efficiency DH5α Competent Cells:

• Designed for general, everyday use
• Contain genetic markers useful for general applications

Ideal for routine subcloning procedures
Subcloning Efficiency DH5α Competent Cells are recommended for routine subcloning of genes into plasmid vectors but are not suitable for the generation of cDNA libraries. These economical cells yield >1 x 10⁶ transformants/µg control DNA per 50 µL reaction.

Flexible cloning capabilities
Subcloning Efficiency DH5α Competent Cells contain the following genetic markers resulting in these benefits:

• lacZΔM15 for blue/white color screening of colonies on plates containing X-gal or Bluo-gal
• recA1 ensures increased insert stability and prevents unwanted recombination
• endA1 improves the yield and quality of plasmid DNA prepared from minipreps
• DH5α competent cells support replication of M13mp vectors but do not support plaque formation

Genotype: F^- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(r_k^-, m_k⁺) phoA supE44 thi-1 gyrA96 relA1 λ^-