Invitrogen™
Subcloning Efficiency™ DH5α Competent Cells
Description
Subcloning Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that provide a transformation efficiency of > 1 x 106 cfu/µg plasmid DNA. Subcloning Efficiency DH5α Competent Cells are an economical solution for routine subcloning procedures or any application where the starting DNA is not limiting.Features of Subcloning Efficiency DH5α Competent Cells:
• Designed for general, everyday use
• Contain genetic markers useful for general applications
Ideal for routine subcloning procedures
Subcloning Efficiency DH5α Competent Cells are recommended for routine subcloning of genes into plasmid vectors but are not suitable for the generation of cDNA libraries. These economical cells yield >1 x 106 transformants/µg control DNA per 50 µL reaction.
Flexible cloning capabilities
Subcloning Efficiency DH5α Competent Cells contain the following genetic markers resulting in these benefits:
• lacZΔM15 for blue/white color screening of colonies on plates containing X-gal or Bluo-gal
• recA1 ensures increased insert stability and prevents unwanted recombination
• endA1 improves the yield and quality of plasmid DNA prepared from minipreps
• DH5α competent cells support replication of M13mp vectors but do not support plaque formation
Genotype: F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 λ-
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Bacterial or Yeast Strain: | DH5α |
|---|---|
| Blue/White Screening: | Yes |
| Cloning Methylated DNA: | No |
| Cloning Unstable DNA: | Not Suitable for cloning unstable DNA |
| Competent Cell Type: | Chemically Competent |
| F'episome (to make ssDNA): | Lacks F' Episome |
| High-throughput Compatibility: | Not High-throughput Compatible (Manual) |
| Preparing Unmethylated DNA: | Not Suitable for preparing unmethylated DNA |
| Product Line: | DH5a, Subcloning Efficiency |
| Propagating ccdB Vectors: | Not for ccdB vector propagation |
| Reduces Recombination: | Yes |
| Shipping Condition: | Dry Ice |
| T1 Phage - Resistant (tonA): | No |
| Transformation Efficiency: | > 1x10^6 |
| Transformation Efficiency Level: | Subcloning Efficiency (10^6-10^7 cfu⁄µg) |
| Species: | E. coli |
| Format: | Tube(s) |
| Improves Plasmid Quality: | Yes |
Contents & storage
Contains:
• Subcloning Efficiency DH5α Competent Cells: 4 vials, 500 µl each (total of 2 ml)
• pUC19 DNA (100 pg/ul): 1 vial, 20 µl
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C.
• Subcloning Efficiency DH5α Competent Cells: 4 vials, 500 µl each (total of 2 ml)
• pUC19 DNA (100 pg/ul): 1 vial, 20 µl
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C.
