Subcloning Efficiency™ DH5α™ Competent Cells

Catalog number: 18265017

Invitrogen™  Related applications: Transformation

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Description

Subcloning Efficiency™ DH5α™ Competent Cells are a versatile strain of chemically competent cells that provide a transformation efficiency of > 1 x 106 cfu/µg plasmid DNA. Subcloning Efficiency™ DH5α™ Competent Cells are an economical solution for routine subcloning procedures or any application where the starting DNA is not limiting.

Features of Subcloning Efficiency™ DH5α™ Competent Cells:

• Designed for general, everyday use
• Contain genetic markers useful for general applications

Ideal for routine subcloning procedures
Subcloning Efficiency™ DH5α™ Competent Cells are recommended for routine subcloning of genes into plasmid vectors but are not suitable for the generation of cDNA libraries. These economical cells yield >1 x 106 transformants/µg control DNA per 50 µL reaction.

Flexible cloning capabilities
Subcloning Efficiency™ DH5α™ Competent Cells contain the following genetic markers resulting in these benefits:

lacZΔM15 for blue/white color screening of colonies on plates containing X-gal or Bluo-gal
recA1 ensures increased insert stability and prevents unwanted recombination
endA1 improves the yield and quality of plasmid DNA prepared from minipreps
• DH5α™ competent cells support replication of M13mp vectors but do not support plaque formation

Genotype: F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 λ-

Specifications

Product Size: -
Amperage: 8A
Capacity: 4.7 cu. ft., 133L
Cabinet Material: High-impact, powder-coated paint
Breaker: 15A
Capacity (English): 4.7 cu. ft.
Capacity (Metric): 133L
Chamber Material: High-impact, powder-coated paint
Casters: 2 in. (5cm) casters (two locking, two Nonlocking), factory installed
Defrost: Automatic
Depth (English) Interior: 20.5 in.
Depth (Metric) Interior: 52.1cm
Door Count: 1
Door Style: Solid
Exterior Finish: high-impact powder paint coating
Dimensions (L x W x H) Exterior: 26 x 24 x 34 in. (66 x 61 x 86.4cm)
Dimensions (D x W x H) Interior: 20.5 x 20 x 20 in. (52.1 x 50.8 x 50.8cm)
Height (English) Interior: 20 in.
Height (English) Exterior: 34 in.
Height (Metric) Exterior: 86.4cm
Height (Metric) Interior: 50.8cm
Hertz: 60Hz
Length (English) Exterior: 26 in.
Interior Finish: high-impact powder paint coating
Insulation: CFC-free urethane foam insulation
Length (Metric) Exterior: 66cm
Plug Type: 5-15P
Ports: 1 in. (2.5cm) access port
Shipping Weight (English): 220 lb.
Shelves: 3
Refrigerant: CFC-free
Shipping Weight (Metric): 100kg
Temperature Control: Microprocessor
Temperature Range (Metric): 1° to 8°C
Voltage: 115V
Width (English) Exterior: 24 in.
Width (English) Interior: 20 in.
Width (Metric) Exterior: 61cm
Doors: Single
Width (Metric) Interior: 50.8cm
Electrical Requirements: 115V 60Hz

Contents & storage

Contains:
• Subcloning Efficiency™ DH5α™ Competent Cells: 4 vials, 500 µl each (total of 2 ml)
• pUC19 DNA (100 pg/ul): 1 vial, 20 µl

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C.

Documents

Manuals & protocols

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