Invitrogen™

MAX Efficiency™ DH5αF´IQ Competent Cells

Catalog number:  18288019

Related applications:

Transformation

Back to top

Description

MAX Efficiency DH5αF´IQ Chemically Competent Cells are high-efficiency chemically competent cells available for M13 cloning, cloning into plasmids with an f1-like origin of replication (phagemids), or cloning into any vector that uses expression from the lac promoter.
MAX Efficiency DH5αF´IQ cells offer the following features:

• >3 x 108 transformants/µg efficiency
• Stable F´ episome eliminates need for growth on minimal media
• ZΔM15 for blue/white screening of colonies or plaques

Three milliliters of lawn cells and a vial of monomer M13mp18 RF DNA are supplied as a control.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain: DH5α
Blue/White Screening: Yes
Cloning Methylated DNA: No
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Competent Cell Type: Chemically Competent
F'episome (to make ssDNA): Contains F' Episome
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Line: DH5a, MAX Efficiency™
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: Yes
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): No
Transformation Efficiency: > 1x10^8
Transformation Efficiency Level: Medium Efficiency (10^8-10^9 cfu⁄µg)
Format: Tube(s)
Improves Plasmid Quality: Yes
Species: E. coli

Contents & storage

Store in ultra-cold freezer (-68 to -85°C).