Invitrogen™
MAX Efficiency™ DH5αF´IQ Competent Cells
Description
MAX Efficiency DH5αF´IQ Chemically Competent Cells are high-efficiency chemically competent cells available for M13 cloning, cloning into plasmids with an f1-like origin of replication (phagemids), or cloning into any vector that uses expression from the lac promoter.MAX Efficiency DH5αF´IQ cells offer the following features:
• >3 x 108 transformants/µg efficiency
• Stable F´ episome eliminates need for growth on minimal media
• ZΔM15 for blue/white screening of colonies or plaques
Three milliliters of lawn cells and a vial of monomer M13mp18 RF DNA are supplied as a control.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Bacterial or Yeast Strain: | DH5α |
|---|---|
| Blue/White Screening: | Yes |
| Cloning Methylated DNA: | No |
| Cloning Unstable DNA: | Not Suitable for cloning unstable DNA |
| Competent Cell Type: | Chemically Competent |
| F'episome (to make ssDNA): | Contains F' Episome |
| High-throughput Compatibility: | Not High-throughput Compatible (Manual) |
| Preparing Unmethylated DNA: | Not Suitable for preparing unmethylated DNA |
| Product Line: | DH5a, MAX Efficiency™ |
| Propagating ccdB Vectors: | Not for ccdB vector propagation |
| Reduces Recombination: | Yes |
| Shipping Condition: | Dry Ice |
| T1 Phage - Resistant (tonA): | No |
| Transformation Efficiency: | > 1x10^8 |
| Transformation Efficiency Level: | Medium Efficiency (10^8-10^9 cfu⁄µg) |
| Format: | Tube(s) |
| Improves Plasmid Quality: | Yes |
| Species: | E. coli |
Contents & storage
Store in ultra-cold freezer (-68 to -85°C).
