ElectroMAX™ DH10B™ Cells

Catalog number: 18290015

Invitrogen™  Related applications: Transformation

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Description

ElectroMAX™ DH10B™ Cells are electrocompetent E. coli cells offering the highest transformation efficiencies of >1 x 1010 cfu/µg plasmid DNA. ElectroMAX™ DH10B™ Cells are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. ElectroMAX™ DH10B™ Cells provide:

• High-efficiency transformation to maximize clones
• Enhanced genetic markers for cDNA or gDNA cloning capabilities

Highest transformation efficiencies
ElectroMAX™ DH10B™ Cells offer the highest transformation efficiencies available with >1 x 1010 transformants/µg of control DNA in a 20 µL reaction. These high transformation efficiencies make ElectroMAX™ DH10B™ Cells ideal for efficient cloning of both prokaryotic and eukaryotic genomic DNA and efficient plasmid rescue from eukaryotic genomes. ElectroMAX™ DH10B™ Cells are also suitable for construction of gene banks, for generation of cDNA libraries using plasmid-derived vectors, and for situations when there is limited amount of DNA.

Note: A high-voltage electroporation apparatus capable of generating field strengths of 16 kV/cm is required.

Versatile cloning capabilities
ElectroMAX™ DH10B™ Cells have the following features:

• Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-gal or Bluo-gal
mcrA genotypic marker and the mcrBC, mrr deletion allows for cloning DNA that contains methylcytosine and methyladenine
endA1 mutation enables increased plasmid yield and quantity

Genotype: F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara, leu)7697 galU galK λ-rpsL nupG

Find the strain and format that you need
DH10B™ cells are available in both electrocompetent and chemically competent formats. Our ElectroMax™ DH10B™ T1R Cells and MegaX DH10B™ T1R Electrocomp Cells offer the benefit of T1 and T5 phage resistance.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Competent Cell Type: Electrocompetent
Species: E. coli
Format: Tube(s)
Transformation Efficiency: > 1x10^10
Bacterial or Yeast Strain: DH10B™
Plasmid Type: May be used for plasmids > 20 kb
Product Size: 0.5 mL
Blue-White Screening: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Reduces Recombination: Yes
Cloning Methylated DNA: Yes
Improves Plasmid Quality: Yes
Propagating ccdB Vectors: Not for ccdB vector propagation
F' Episome (to make ssDNA): Lacks F' Episome
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA): No
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Shipping Condition: Dry Ice

Contents & storage

Contains:
• ElectroMAX™ DH10B™ Cells: 5 vials, 100 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 2 bottles, 6 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

Documents

Manuals & protocols

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