Invitrogen™

ElectroMAX™ DH10B Cells

Catalog number:  18290015

 Related applications:

Transformation

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Description

ElectroMAX DH10B Cells are electrocompetent E. coli cells offering the highest transformation efficiencies of >1 x 1010 cfu/µg plasmid DNA. ElectroMAX DH10B Cells are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. ElectroMAX DH10B Cells provide:

• High-efficiency transformation to maximize clones
• Enhanced genetic markers for cDNA or gDNA cloning capabilities

Highest transformation efficiencies
ElectroMAX DH10B Cells offer the highest transformation efficiencies available with >1 x 1010 transformants/µg of control DNA in a 20 µL reaction. These high transformation efficiencies make ElectroMAX DH10B Cells ideal for efficient cloning of both prokaryotic and eukaryotic genomic DNA and efficient plasmid rescue from eukaryotic genomes. ElectroMAX DH10B Cells are also suitable for construction of gene banks, for generation of cDNA libraries using plasmid-derived vectors, and for situations when there is limited amount of DNA.

Note: A high-voltage electroporation apparatus capable of generating field strengths of 16 kV/cm is required.

Versatile cloning capabilities
ElectroMAX DH10B Cells have the following features:

• Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-gal or Bluo-gal
mcrA genotypic marker and the mcrBC, mrr deletion allows for cloning DNA that contains methylcytosine and methyladenine
endA1 mutation enables increased plasmid yield and quantity

Genotype: F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara, leu)7697 galU galK λ-rpsL nupG

Find the strain and format that you need
DH10B cells are available in both electrocompetent and chemically competent formats. Our ElectroMax DH10B T1R Cells and MegaX DH10B T1R Electrocomp Cells offer the benefit of T1 and T5 phage resistance.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Competent Cell Type: Electrocompetent
Species: E. coli
Format: Tube(s)
Transformation Efficiency: > 1x10^10
Bacterial or Yeast Strain: DH10B
Product Line: DH10B, ElectroMAX™
Blue-White Screening: Yes
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
F' Episome (to make ssDNA): Lacks F' Episome
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Improves Plasmid Quality: Yes
Plasmid Type: May be used for plasmids > 20 kb
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Size: 100 µL
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: Yes
T1 Phage - Resistant (tonA): No
Shipping Condition: Dry Ice

Contents & storage

Contains:
• ElectroMAX DH10B Cells: 5 vials, 100 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 2 bottles, 6 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

Documents

Manuals & protocols