Invitrogen™

MAX Efficiency™ DH10B Competent Cells

Catalog number: 18297010
Invitrogen™

MAX Efficiency™ DH10B Competent Cells

Catalog number: 18297010
Catalog Number
18297010
Unit Size
5 x 200 µL
Price (USD)
167.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
182970105 x 200 µL
167.00
Your Price:
***
Product Overview
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MAX Efficiency DH10B Competent Cells are highly efficient E. coli, perfect for most applications. DH10B E. coli are available in both electrocompetent and chemically competent formats. The high transformation efficiency makes them ideal for generating cDNA or genomic libraries. High-efficiency transformation is seen with plasmids 150 kb in size. The versatile DH10B genotype has the following features:

lacZ for blue/white screening of recombinant clones
• Elimination of mcrA, mcrBC, mrr, and hsdRMS restriction systems to allow construction of more representative genomic libraries
• A1 mutation to increase plasmid yield and quantity
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain
DH10B
Blue/White Screening
Yes
Cloning Methylated DNA
Yes
Cloning Unstable DNA
Not Suitable for cloning unstable DNA
Competent Cell Type
Chemically Competent
F'episome (to make ssDNA)
Lacks F' Episome
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
Propagating ccdB Vectors
Not for ccdB vector propagation
Reduces Recombination
Yes
Shipping Condition
Dry Ice
T1 Phage - Resistant (tonA)
No
Transformation Efficiency
> 1x10^9
Transformation Efficiency Level
High Efficiency (> 10^9 cfu⁄µg)
Format
Tube(s)
Product Line
DH10B, MAX Efficiency™
Product Type
Competent Cells
Species
E. coli
Improves Plasmid Quality
Yes

Contents & Storage

Contains:
• MAX Efficiency DH10B Competent Cells: 5 vials, 200 µl each (total of 1 ml)
• pUC19 DNA (0.01 µg/ml): 1 vial, 100 µl
• S.O.C. medium: 2 bottles, 6 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

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