Invitrogen™

ElectroMAX™ DH12S Cells

Catalog number:  18312017

Related applications:

Transformation

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Description

ElectroMAX DH12S Cells, a derivative of DH10B are suitable for transformation by electroporation (1,2). They may be used for production of single-stranded DNA and in procedures requiring high transformation efficiencies, such as generation of cDNA, genomic, and subtractive cDNA libraries. The DH12S strain is endA+, which allows the production of ssDNA with less contaminating double-stranded DNA. The lacIq on the F´ marker allows for regulatable expression from the lac promoter. Unlike most E. colihosts used in cloning genomic DNA (3), the systems that restrict DNAs containing methylated cytosine and adenine residues (mcrA, mcrB, mcrC, and mrr) have been eliminated in
the DH12S strain, allowing the construction of more representative genomic libraries and more efficient plasmid rescue procedures (4,5). M13KO7 helper phage is also supplied for the production of ss DNA. ElectroMAX DH12S Cells offer:

• >1 x 1010 transformants/µg efficiency with electroporation for unsurpassed cloning results
• A unique genotype to support production of exceptionally clean single stranded DNA
• Stable F´ episome to eliminate the need for growth on minimal media
• Better sequence representation eliminating mcrA, mcrBC, mrr, hsdRMS restriction systems
• Blue/white screening of recombinant clones due to lacZΔM15
• Ready-to-use M13KO7 helper phage for single stranded DNA production
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain: DH12S
Blue/White Screening: Yes
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Competent Cell Type: Electrocompetent
F'episome (to make ssDNA): Contains F' Episome
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Plasmid: May be used for plasmids > 20 kb
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Line: DH12S, ElectroMAX™
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: Yes
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): No
Transformation Efficiency: > 1x10^10
Transformation Efficiency Level: High Efficiency (> 10^9 cfu⁄µg)
Format: Tube(s)
Improves Plasmid Quality: No
Species: E. coli

Contents & storage

Store in ultra-cold freezer (-68 to -85°C).