Lipofectamine™ Transfection Reagent
Lipofectamine™ Transfection Reagent
인용 및 참조 문헌 (71)
Invitrogen™

Lipofectamine™ Transfection Reagent

Lipofectamine™ Transfection Reagent는 DNA을 진핵세포에 transfection하도록 만들어진 본사 제1세대 cationic-lipid transfection reagent입니다. Lipofectamine™ Transfection Reagent는 17년간 수 천편의 논문에 인용되고자세히 알아보기
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카탈로그 번호수량
183240204 mL
183240100.3 mL
183240121 mL
카탈로그 번호 18324020
제품 가격(KRW)
2,269,000
Each
카트에 추가하기
수량:
4 mL
제품 가격(KRW)
2,269,000
Each
카트에 추가하기
Lipofectamine™ Transfection Reagent는 DNA을 진핵세포에 transfection하도록 만들어진 본사 제1세대 cationic-lipid transfection reagent입니다. Lipofectamine™ Transfection Reagent는 17년간 수 천편의 논문에 인용되고 많은 cell line에 사용된 믿을 수 있는 우수한 reagent입니다.

Lipofectamine™ reagent 특징:
• high-throughput application 작업에 입증된 reagent
• stable cell line 수립에 믿을 수 있는 reagent
• Protein expression의 레벨을 높이기 위해 PLUS™ reagent와 함께 사용할 수 있는 reagent

입증된 기술
라이프 테크놀로지스의 Lipofectamine™ brand reagent은 가장 인용이 많이 된 transfection reagent family로 1993년 출시된 이후 45,000번 이상 인용되었습니다. Lipofectamine™ Transfection Reagent는 예외없이 다양한 cell line에 한결같은 성능을 제공하며 PLUS™ Reagent와 함께 사용 시 BHK-21, NIH 3T3, COS-1, Fibroblasts, Keratinocytes, HT-29, MRC-5, SK-BR3 같은 세포에 우수한 성능을 보였습니다.

A “Value” Plasmid DNA 대안
이런 레거시로 모든 과학자와 실험실에 이들의 자본력과 무관하게 최고의 reagent 제공을 약속합니다. Lipofectamine™ Transfection Reagent는 간편한 프로토콜로 진핵세포의 plasmid DNA transfection을 대신할 수 있는 제품입니다. 다양한 plasmid DNA transfection 조건에서 최적의 결과를 얻으려면 잠재적인 독성을 비롯해 세포독성의 가능성을 최소화한 Lipofectamine™ LTX Reagent을 사용할 것을 권장합니다. 다양한 cell line transfection이나 RNA transfection에는 Lipofectamine™ 2000을 권장합니다.

이 제품은 연구용으로만 사용가능합니다. 치료 또는 진단 목적으로 동물이나 인간에 사용할 수 없습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(애플리케이션)Transfection
고처리량 호환성Not High-throughput Compatible (Manual)
제품라인Lipofectamine
제품 유형Transfection Reagent
수량4 mL
혈청 호환 가능No
배송 조건Wet Ice
셀 유형Established Cell Lines, Primary Cells, Hard-to-Transfect Cells
형식6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
샘플 종류Plasmid DNA
Transfection TechniqueLipid-based Transfection
Unit SizeEach
구성 및 보관
Contains 4 vial (1 mL each) of Lipofectamine™ Transfection Reagent. Store at 2°C to 8°C. Do not freeze.

자주 묻는 질문(FAQ)

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

What is the difference between reverse transfection and forward transfection? What should I use?

In forward transfection, cells are seeded to appropriate confluence or cell density in wells or dishes, and the lipid-DNA complexes are added the next day. In reverse transfection, the transfection complexes are prepared inside the wells, after which cells and medium are added. Reverse transfection is faster to perform than forward transfection, and is the method of choice for high-throughput transfection. For non-high-throughput transfections, generally forward transfections have better efficiency for most cell types.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Is there a place where I can find references from other researchers who have used your transfection reagents?

Visit the product page for each reagent type and you will see a list of references at the bottom of the page. A table that lists specific cell line references is also accessible. We also recommend www.highwire.org as a search engine to find a large selection of up-to-date research articles using our transfection products. Simply include the name of the transfection reagent and your cell line/application of interest in your search criteria.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use antibiotics in the medium during transfection?

Antibiotics can be used in the medium for culturing of cell lines. However, we do not recommend using antibiotics in the transfection medium unless previously tested in the cell type and payload being transfected. This is because presence of antibiotics during transfection may adversely affect transfection efficiency (i.e., positively charged antibiotics binding to the DNA being transfected) and overall health of cells being transfected.

For stable transfection, we recommend waiting wait 24-48 hrs after transfection before adding selected antibiotics.

Find additional tips, troubleshooting help, and resources within ourTransfection Basics Support Center.

Is it necessary to use serum-free medium during lipid transfection?

It is not necessary to use serum-free medium during lipid transfection. However, it is critical to form the lipid:nucleic acid complex in the absence of serum, because proteins can interfere with complex formation. Once the complexes are formed, they can be added to cells in serum-containing medium. For optimal results with Lipofectin Transfection Reagent, we recommend performing transfection in medium without serum.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

View all Lipofectamine™ Transfection Reagent, 4 mL FAQs

인용 및 참조 문헌 (71)

인용 및 참조 문헌
Abstract
Low-density lipoprotein receptor-related protein 5 (LRP5) is essential for normal cholesterol metabolism and glucose-induced insulin secretion.
Authors:Fujino T, Asaba H, Kang MJ, Ikeda Y, Sone H, Takada S, Kim DH, Ioka RX, Ono M, Tomoyori H, Okubo M, Murase T, Kamataki A, Yamamoto J, Magoori K, Takahashi S, Miyamoto Y, Oishi H, Nose M, Okazaki M, Usui S, Imaizumi K, Yanagisawa M, Sakai J, Yamamoto TT,
Journal:Proc Natl Acad Sci U S A
PubMed ID:12509515
A Wnt coreceptor low-density lipoprotein receptor-related protein 5 (LRP5) plays an essential role in bone accrual and eye development. Here, we show that LRP5 is also required for normal cholesterol and glucose metabolism. The production of mice lacking LRP5 revealed that LRP5 deficiency led to increased plasma cholesterol levels in ... More
Regulation of cortical dendrite development by Slit-Robo interactions.
Authors:Whitford Kristin L; Marillat Valérie; Stein Elke; Goodman Corey S; Tessier-Lavigne Marc; Chédotal Alain; Ghosh Anirvan;
Journal:Neuron
PubMed ID:11779471
Slit proteins have previously been shown to regulate axon guidance, branching, and neural migration. Here we report that, in addition to acting as a chemorepellant for cortical axons, Slit1 regulates dendritic development. Slit1 is expressed in the developing cortex, and exposure to Slit1 leads to increased dendritic growth and branching. ... More
Extracellular Export of Sphingosine Kinase-1 Enzyme. SPHINGOSINE 1-PHOSPHATE GENERATION AND THE INDUCTION OF ANGIOGENIC VASCULAR MATURATION.
Authors:Ancellin Nicolas; Colmont Chantal; Su Joseph; Li Qin; Mittereder Nanette; Chae Sung-Suk; Stefansson Steingrimur; Liau Gene; Hla Timothy;
Journal:J Biol Chem
PubMed ID:11741921
The enzyme sphingosine kinase (SK) catalyzes the formation of sphingosine 1-phosphate (S1P), a bioactive lipid that acts extracellularly on G protein-coupled receptors of the S1P(1)/EDG-1 subfamily. Although S1P is formed in the cytosol of various cells, S1P release is not understood and is controversial because this lipid mediator is also ... More
Different composite regulatory elements direct expression of the human alpha subunit gene to pituitary and placenta.
Authors: Heckert L L; Schultz K; Nilson J H;
Journal:J Biol Chem
PubMed ID:7592867
'To identify elements of the human alpha subunit gene necessary for cell-specific expression, we generated an array of block mutations spanning approximately 400 base pairs (bp) of promoter proximal region and examined them using transient transfection analysis in pituitary (alpha T3) and placental (BeWo) cell lines. Comparison of promoter activity ... More
ATF3 gene. Genomic organization, promoter, and regulation.
Authors: Liang G; Wolfgang C D; Chen B P; Chen T H; Hai T;
Journal:J Biol Chem
PubMed ID:8576171
'ATF3 gene, which encodes a member of the activating transcription factor/cAMP responsive element binding protein (ATF/CREB) family of transcription factors, is induced by many physiological stresses. As a step toward understanding the induction mechanisms, we isolated the human ATF3 gene and analyzed its genome organization and 5''-flanking region. We found ... More
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