Oligo(dT)₂₀ Primer

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Invitrogen™

Oligo(dT)₂₀ Primer

≥50°C 온도에서 역전사효소를 이용한 first-strand cDNA 합성에 사용되는 oligo(dT)20 primer는 20 deoxythymidylic acid 잔기 스트링으로 mRNA의 poly(A) tail에 부착됩니다. Oligo(dT)20는자세히 알아보기

카탈로그 번호	수량
18418020	50 μL

카탈로그 번호 18418020

제품 가격(KRW)

172,000

온라인 행사

Ends: 31-Dec-2025

191,000

할인액 19,000 (10%)

카트에 추가하기

50 μL

제품 가격(KRW)

172,000

온라인 행사

Ends: 31-Dec-2025

191,000

할인액 19,000 (10%)

카트에 추가하기

≥50°C 온도에서 역전사효소를 이용한 first-strand cDNA 합성에 사용되는 oligo(dT)₂₀ primer는 20 deoxythymidylic acid 잔기 스트링으로 mRNA의 poly(A) tail에 부착됩니다. Oligo(dT)₂₀는 SuperScript™ III Reverse Transcriptase, ThermoScript™ Reverse Transcriptase, 또는 Cloned AMV Reverse Transcriptase와 함께 사용하도록 권장됩니다.

품질 관리: 이 제품은 first-strand cDNA 합성 반응으로 적격성을 평가합니다.

For Research Use Only. Not for use in diagnostic procedures.

5'프라이머 수정Phosphate

용도(애플리케이션)First-strand cDNA Synthesis

형태Liquid

프라이머 길이20-mer

프라이머 서열5'd PO4 [(T)20]3'

제품 유형Primer

정제 방법Gel-purified, Desalted

수량50 μL

배송 조건Approved for shipment on Wet or Dry Ice

농도50 μM

PrimerOligo dT

Unit SizeEach

구성 및 보관

• Oligo(dT)₁₆ Primer (50 μL at 50 μM)

Store at –15 to –25°C.
Aliquot to avoid repeated freezing and thawing.

자주 묻는 질문(FAQ)

Which components of the SuperScript III First Strand Synthesis System for RT-PCR are available for purchase separately?

The following components are available as stand-alone items:

- Superscript III Reverse Transcriptase (Cat. Nos. 18080093, 18080044, 18080085)
- Oligo (dT)20 Primer (Cat. No. 18418020)
- Random hexamers (Cat. No. 48190011)
- 10 mM dNTP Mix (Cat. Nos. 18427013, 18427088)
- RNAseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)
- E. coli RNAse H (Cat. Nos. 18021014, 18021071)

How does the Anchored Oligo(dT)20 Primer differ from standard oligo(dT) primers?

Anchored Oligo(dT) primers have 2 random bases at the 3' end of the stretch of Ts. The first random base is either an A, G, or C, while the second can be any of the 4 standard nucleotides, i.e., A, T, G or C. The use of anchored oligo(dT) primers results in increased cDNA synthesis yields.

For first-strand cDNA synthesis, is it better to use oligo(dT), random hexamers, gene-specific primer (GSP), or combination of these primers?

The choice of primer depends on your experimental goals. Oligo(dT) is recommended when using total RNA for cDNA synthesis. It is the key to full-length cDNA synthesis. Random hexamers give a series of short first-strand products spanning the entire mRNA. Use of random hexamers may be helpful if the PCR fragment is at the 5´ end of a large mRNA. To ensure full-length cDNA synthesis of large transcripts, oligo(dT) can be added along with random hexamers during first-strand synthesis. Gene-specific primers (GSP) for cDNA synthesis may also be used and are required in a few applications such as 5´ RACE and qRT-PCR. For GC-rich templates or templates rich in secondary structure, a GSP may not work as well as priming with oligo dT for first-strand synthesis. If an RT-PCR is problematic, trying different options of oligo dT, random primers and/or GSP for priming first-strand synthesis may resolve the issue. Oligo(dT)20 primer (Cat. No. 18418-020) is recommended for use with SuperScript III Reverse Transcriptase (Cat. no. 18080-044), ThermoScript Reverse Transcriptase (Cat. No. 12236-014), Thermo-X Reverse Trascriptase (Cat. No. 11150-025), and Cloned AMV Reverse Transcriptase (Cat. No. 12328-019).

Reference:
Frohman,M.A., Dush,M.K., Martin, G.R. (1988) Proc. Nat. Acad. Sci USA 85, 8998