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Invitrogen™
SuperScript V Reverse Transcriptase
Invitrogen™ SuperScript™ V Reverse Transcriptase (RT) is a next-generation MMLV reverse transcriptase designed for fast, reliable first-strand cDNA synthesis.
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| Catalog Number | Quantity |
|---|---|
| 19090050 | 10,000 Units |
| 19090010 | 2000 Units |
| 19090200 | 40,000 Units |
Catalog number 19090050
Price (USD)
374.00
Each
Quantity:
10,000 Units
Price (USD)
374.00
Each
Invitrogen™ SuperScript™ V Reverse Transcriptase (RT) is a next-generation MMLV reverse transcriptase designed for fast, reliable first-strand cDNA synthesis. Building on the advanced performance of the SuperScript™ IV reverse transcriptase, SuperScript V RT combines high sensitivity, thermostability, and processivity with improved resistance to oxidation and greater workflow flexibility.
SuperScript V RT supports reliable cDNA synthesis from low-input RNA as well as highly structured, GC-rich, degraded, and inhibitor-containing samples.
Features of SuperScript V Reverse Transcriptase include:
- Broad RNA input range—supports cDNA synthesis from 100 fg to 5 μg of RNA
- Robust performance with challenging RNA—reaction temperatures up to 65°C help transcribe highly structured and GC-rich RNA
- Fast cDNA synthesis—complete first-strand cDNA synthesis in as little as 1–10 minutes
- Improved resistance to oxidation— enhanced reagent stability supports ambient shipping and room-temperature storage for up to 6 months
- Flexible workflow—room-temperature reaction setup and stability of assembled reactions for up to 24 hours
SuperScript V RT is well suited for RT-PCR, RT-qPCR, gene expression analysis, and other applications requiring high-quality first-strand cDNA synthesis. Its enhanced reagent and assembled-reaction stability provides additional flexibility for routine, high-throughput, and automated workflows.
A DTT-free, animal origin–free formulation combined with reduced cold chain requirements further simplifies reagent handling and supports more sustainable workflows.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatStand-alone Enzyme
Inhibitor Tolerance (Simple)High
No. of Reactions50 Reactions
Optimal Reaction Temperature50°C
Product TypeReverse Transcription Reagent
Quantity10,000 Units
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Reverse TranscriptaseSuperScript V
Ribonuclease H ActivityReduced
Sensitivity0.1 pg total RNA
Shipping ConditionDry Ice, Wet Ice, Ambient
Size (Final Product)Up to 20 kb
Starting MaterialRNA
TechniqueReverse Transcription
ThermostabilityUp to 65°C
Concentration200 U/μL
Detection MethodqPCR, NGS, PCR
For Use With (Application)cDNA Synthesis, RT-PCR, Real Time PCR (qPCR), Sequencing, Next-Generation Sequencing (NGS)
FormLiquid
GC-Rich PCR PerformanceHigh
Label or DyeNot Applicable
PCR Method2-step RT-qPCR, RT-PCR
Reaction Speed10 min.
Unit SizeEach
Contents & Storage
• SuperScript V Reverse Transcriptase (50 μL)
• 5X SuperScript V Reaction Buffer (500 μL)
Store all contents at:
• -20°C for long-term storage until expiry date
• +5°C for up to 12 months
• Room temperature for up to 6 months
• 5X SuperScript V Reaction Buffer (500 μL)
Store all contents at:
• -20°C for long-term storage until expiry date
• +5°C for up to 12 months
• Room temperature for up to 6 months