CaptureSelect™ C-tag Affinity Matrix

Catalog number: 1913070500

 Related applications: Chromatography | Protein Purification & Isolation

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Description

The CaptureSelect™ C-tag Affinity Matrix combines unique selectivity for a small 4-amino acid peptide tag (E-P-E-A, glutamic acid-proline-glutamic acid-alanine) with the benefits of a robust and high quality affinity matrix. The CaptureSelect™ C-tag Affinity Matrix purifies C-terminal tagged proteins with high affinity and selectivity, even in the presence of urea and guanidine HCl, from complex mixtures like cytoplasm or periplasmatic fractions in a one-step process. Mild elution conditions at neutral pH can be applied using magnesium chloride or propylene glycol, which ensures high activity recoveries of pH-sensitive target proteins. The affinity resin recognizes the E-P-E-A tag sequence when fused either directly to the C-terminus of a protein or through linkers between the C-terminus and the E-P-E-A tag.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Column Type: Bulk Resin
Format: Bulk Media
Immobilized Phase: Anti C-tag
Product Size: 500 mL

Contents & storage

Store at 2–8°C.

Documents

Manuals & protocols