The CaptureSelect™ KappaXL Affinity Matrix has been specifically designed for the purification of recombinant human Ig’s (including IgA, IgM, IgD, and IgE) containing a Kappa light chain and their Fab fragments.
Features of this affinity matrix include:
• Mild elution for Fab fragments and antibodies • Human specific, no binding to bovine antibodies, for example • Excellent scalability • Non-animal-derived
CaptureSelect™ KappaXL Affinity Matrix purifies Ig’s, Fab, and Fab2 fragments directly from complex source materials in a single step with high purity and yield.
Free of Animal Components Our CaptureSelect™ products are affinity ligands created by a proprietary technology based on Camelid-derived single domain antibody fragments. The ligand is a 13 kDa single domain fragment comprising the 3 CDRs that form the antigen binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.
Matrix: agarose-based, aldehyde-activated Average particle size: 65 ± 10 µm Ligand: CaptureSelect™ KappaXL affinity ligand Ligand coupling method: aldehyde coupling via NH2 residues of the ligand Binding capacity: 29–38 g IgG/L resin depending on flow rate, column height, and contact time Elution conditions: 20 mM citric acid, pH 3.5 Flow characteristics: 200–400 cm/h (up to 1 bar) Formulation buffer: 20%(v/v) ethanol
Caution: For manufacturing, processing, or repacking.