Thermo Scientific™

CaptureSelect™ KappaXL Affinity Matrix

Catalog number:  19432101L

Related applications:

Protein Biology

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Description

The CaptureSelect™ KappaXL Affinity Matrix has been specifically designed for the purification of recombinant human Ig’s (including IgA, IgM, IgD, and IgE) containing a Kappa light chain and their Fab fragments.

Features of this affinity matrix include:

• Mild elution for Fab fragments and antibodies
• Human specific, no binding to bovine antibodies, for example
• Excellent scalability
• Non-animal-derived

CaptureSelect™ KappaXL Affinity Matrix purifies Ig’s, Fab, and Fab2 fragments directly from complex source materials in a single step with high purity and yield.

Free of Animal Components
Our CaptureSelect™ products are affinity ligands created by a proprietary technology based on Camelid-derived single domain antibody fragments. The ligand is a 13 kDa single domain fragment comprising the 3 CDRs that form the antigen binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main Characteristics:

Matrix: agarose-based, aldehyde-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect™ KappaXL affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: 29–38 g IgG/L resin depending on flow rate, column height, and contact time
Elution conditions: 20 mM citric acid, pH 3.5
Flow characteristics: 200–400 cm/h (up to 1 bar)
Formulation buffer: 20%(v/v) ethanol
Caution: For manufacturing, processing, or repacking.

Specifications

Column Type: Bulk Resin
Product Line: CaptureSelect™
Stationary Phase: Anti LC-kappa
Format: Bottle(s)
Flow Rate: 200 to 400 cm⁄hr
Matrix: Agarose based, aldehyde activated
Particle Size: 65 micron

Contents & storage

Store in refrigerator (2–8°C).