The CaptureSelect™ KappaXL Affinity Matrix has been specifically designed for the purification of recombinant human Ig’s (including IgA, IgM, IgD, and IgE) containing a Kappa light chain and their Fab fragments.
Features of this affinity matrix include:
• Mild elution for Fab fragments and antibodies
• Human specific, no binding to bovine antibodies, for example
• Excellent scalability
CaptureSelect™ KappaXL Affinity Matrix purifies Ig’s, Fab, and Fab2 fragments directly from complex source materials in a single step with high purity and yield.
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Matrix: agarose-based, aldehyde-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect™ KappaXL affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: 29–38 g IgG/L resin depending on flow rate, column height, and contact time
Elution conditions: 20 mM citric acid, pH 3.5
Flow characteristics: 50–200 cm/h (up to 2 bar)
Formulation buffer: 20%(v/v) ethanol