Thermo Scientific™

CaptureSelect™ FcXL Affinity Matrix

Catalog number:  19432801L

Related applications:

Protein Biology

Back to top

Description

CaptureSelect™ FcXL Affinity Matrix has been specifically designed for the purification of recombinant human IgG, Fc fusion proteins, and plasma derived IgG. It recognizes the CH3 domain of all human IgG subclasses (IgG1, 2, 3, and 4).

Features of this affinity matrix include:

• Mild elution, making it suitable for Fc fusion proteins
• Human specific, no binding to bovine antibodies, for example
• Excellent scalability
• Non-animal-derived

CaptureSelect™ FcXL Affinity Matrix purifies IgGs and Fc fusion proteins directly from complex source materials in a single step with high purity and yield.

Free of Animal Components
Our CaptureSelect™ products are affinity ligands created by a proprietary technology based on Camelid-derived single domain antibody fragments. The ligand is a 13 kDa single domain fragment comprising the 3 CDRs that form the antigen binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main Characteristics:

Matrix: agarose-based, aldehyde-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect™ FcXL affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: 25–35 g IgG/L resin depending on flow rate, column height, and contact time
Elution conditions: 20 mM citric acid or acetic acid, pH 3–4, or 20 mM sodium acetate, 1.0 M MgCl2, 40% (v/v) propylene glycol, pH 5–6
Flow characteristics: 150–300 cm/h
Formulation buffer: 20%(v/v) ethanol
Caution: For manufacturing, processing, or repacking.

Specifications

Column Type: Bulk Resin
Product Line: CaptureSelect™
Stationary Phase: Anti IgG-Fc
Format: Bottle(s)
Flow Rate: 200 to 400 cm⁄hr
Matrix: Agarose based, aldehyde activated
Particle Size: 65 micron

Contents & storage

Store in refrigerator (2–8°C).