Thermo Scientific™

CaptureSelect™ FcXL Affinity Matrix

Catalog number: 19432801L
Thermo Scientific™

CaptureSelect™ FcXL Affinity Matrix

Catalog number: 19432801L
Catalog Number
19432801L
Unit Size
1 L
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Availability
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Quantity
-
Catalog Number
Unit Size
5 mL
Price (USD)
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Unit Size
10 mL
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50 mL
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250 mL
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-
Catalog Number
Unit Size
5 L
Price (USD)
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Unit Size
10 L
Price (USD)
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-
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Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
19432801L1 LContact Us ›--
1943280055 mL
-
19432801010 mL
-
19432805050 mL
-
1943280250250 mLContact Us ›--
19432805L5 LContact Us ›--
194328010L10 LContact Us ›--
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CaptureSelect™ FcXL Affinity Matrix has been specifically designed for the purification of recombinant human IgG, Fc fusion proteins, and plasma derived IgG. It recognizes the CH3 domain of all human IgG subclasses (IgG1, 2, 3, and 4).

Features of this affinity matrix include:

  • Mild elution, making it suitable for Fc fusion proteins
  • Human specific, no binding to bovine antibodies, for example
  • Excellent scalability
  • Non-animal-derived

CaptureSelect™ FcXL Affinity Matrix purifies IgGs and Fc fusion proteins directly from complex source materials in a single step with high purity and yield.

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main Characteristics:

  • Matrix: agarose-based, aldehyde-activated
  • Average particle size: 65 ± 10 μm
  • Ligand: CaptureSelect™ FcXL affinity ligand
  • Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
  • Binding capacity: 25–35 g IgG/L resin depending on flow rate, column height, and contact time
  • Elution conditions: 20 mM citric acid or acetic acid, pH 3–4, or 20 mM sodium acetate, 1.0 M MgCl2, 40% (v/v) propylene glycol, pH 5–6
  • Flow characteristics: 50–200 cm/h (up to 2 bar)
  • Formulation buffer: 20%(v/v) ethanol

Specifications

Binding Capacity
25 to 35 g IgG/L
Flow Rate
50 to 200 cm/hr. (Up to 2 bar)
Matrix
Agarose-based, aldehyde-activated
Particle Size
65 ± 10 μm
Form
Agarose Bead
Product Line
CaptureSelect™
Quantity
1 L
For Use With (Application)
IgG purification
Target
Fc of IgG, CH3 domain
Type
Affinity Matrix
Ligand Type
FcXL affinity ligand
Stationary Phase
Anti-IgG-Fc

Contents & Storage

Store in refrigerator (2°C to 8°C).

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