CaptureSelect tPA Affinity Matrix has been specifically designed for the purification of recombinant human tissue plasminogen activator (tPA). This matrix can be used for the purification of recombinant human tPA from cell culture harvests with high purity and yield in a single step. The design of the tPA affinity matrix allows elution of the tPA protein at a neutral pH in the presence of magnesium chloride, but standard low-pH elution buffers like 0.1 M glycine, pH 3.0, can also be used.
Features of this affinity matrix include: • Neutral pH elution with 20 mM Tris, 2.0 M MgCl2, 0.2 M arginine pH 7.0 • Caustic cleaning with 50 mM NaOH • Excellent scalability • Non-animal-derived
Free of animal components CaptureSelect products are affinity ligands created by a proprietary technology based on camelid-derived single-domain antibody fragments. The ligand is a 13 kDa fragment comprising the 3 complementarity-determining regions (CDRs) that form the antigen binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.
Matrix: agarose-based, epoxide-activated Average particle size: 65 ± 10 µm Ligand: CaptureSelect tPA affinity ligand Ligand coupling method: aldehyde coupling via NH2 residues of the ligand Binding capacity: 8-10 g/L depending on flow rate, column height, and contact time Elution conditions: Neutral pH: 20 mM Tris, 2.0 M MgCl2, 0.2 M arginine pH 7.0; Low pH: 0.1 M glycine, pH 3.0Flow characteristics: 200–400 cm/h (up to 1 bar) Formulation buffer: 20% (v/v) ethanol
Caution: For manufacturing, processing, or repacking.