Thermo Scientific™

CaptureSelect™ tPA Affinity Matrix

Catalog number:  1943430250

Related applications:

Chromatography

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Description

CaptureSelect tPA Affinity Matrix has been specifically designed for the purification of recombinant human tissue plasminogen activator (tPA). This matrix can be used for the purification of recombinant human tPA from cell culture harvests with high purity and yield in a single step. The design of the tPA affinity matrix allows elution of the tPA protein at a neutral pH in the presence of magnesium chloride, but standard low-pH elution buffers like 0.1 M glycine, pH 3.0, can also be used.

Features of this affinity matrix include:
• Neutral pH elution with 20 mM Tris, 2.0 M MgCl2, 0.2 M arginine pH 7.0
• Caustic cleaning with 50 mM NaOH
• Excellent scalability
• Non-animal-derived

Free of animal components
CaptureSelect products are affinity ligands created by a proprietary technology based on camelid-derived single-domain antibody fragments. The ligand is a 13 kDa fragment comprising the 3 complementarity-determining regions (CDRs) that form the antigen binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect tPA affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: 8-10 g/L depending on flow rate, column height, and contact time
Elution conditions: Neutral pH: 20 mM Tris, 2.0 M MgCl2, 0.2 M arginine pH 7.0; Low pH: 0.1 M glycine, pH 3.0Flow characteristics: 200–400 cm/h (up to 1 bar)
Formulation buffer: 20% (v/v) ethanol

Caution: For manufacturing, processing, or repacking.

Specifications

Product Line: CaptureSelect™
Shipping Condition: Wet Ice

Contents & storage

Store in refrigerator (2–8°C).