CaptureSelect tPA Affinity Matrix has been specifically designed for the purification of recombinant human tissue plasminogen activator (tPA). This matrix can be used for the purification of recombinant human tPA from cell culture harvests with high purity and yield in a single step. The design of the tPA affinity matrix allows elution of the tPA protein at a neutral pH in the presence of magnesium chloride, but standard low-pH elution buffers like 0.1 M glycine, pH 3.0, can also be used.
Features of this affinity matrix include:
• Neutral pH elution with 20 mM Tris, 2.0 M MgCl2, 0.2 M arginine pH 7.0
• Caustic cleaning with 50 mM NaOH
• Excellent scalability
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect tPA affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: 8-10 g/L depending on flow rate, column height, and contact time
Elution conditions: Neutral pH: 20 mM Tris, 2.0 M MgCl2, 0.2 M arginine pH 7.0; Low pH: 0.1 M glycine, pH 3.0Flow characteristics: 50–200 cm/h (up to 2 bar)
Formulation buffer: 20% (v/v) ethanol