Invitrogen™

Biotin-14-dATP

Catalog number:  19524016

Related applications:

Nucleic Acid Labeling & Oligo Synthesis

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Description

Biotin-14-dATP is a patented dATP analog with biotin attached at the 6-position of the purine base by a 14-atom linker. The long, 14-atom spacer arm allows accessibility to streptavidin for sensitive detection of probe-target hybrids in in situ and filter-based hybridizations. Biotin-14-dATP can be efficiently incorporated into DNA by nick translation in the presence of dCTP, dGTP, and dTTP (1). Biotin-14-dATP can be added to the 3´ end of DNA using terminal deoxynucleotidyl transferase (2). The amount supplied is sufficient for labeling up to 50 µg of DNA by nick translation.

Performance and Quality Testing: Purity is evaluated by reverse-phase HPLC analysis.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label Type: Biotin & Other Haptens
Labeling Method: Direct Labeling
Label or Dye: Biotin
Shipping Condition: Approved for shipment on Wet or Dry Ice
Concentration: 0.4 mM

Contents & storage

Biotin-14-dATP is supplied at 0.4 mM in 100 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Store at -20°C.