Informational:This product will be discontinued in Q3 2022. Please read here.
Thermo Scientific™

POROS™ CaptureSelect™ IgM Affinity Matrix

Catalog number: 195289010
Thermo Scientific™

POROS™ CaptureSelect™ IgM Affinity Matrix

Catalog number: 195289010
Catalog Number
195289010
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10 mL
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Price: 1,872.00
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50 mL
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Product Overview
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Additional Information
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POROS™ CaptureSelect™ IgM Affinity Matrix is intended for the purification of human IgM, with no binding to other immunoglobulins.

Features of this affinity matrix include:
• IgM purification from plasma and serum
• Purification of monoclonal IgM from cell cultures
• Efficient purification of IgM with high yields and retained activity
• Excellent scalability
• Non-animal derived
• High flow rates
• Cross-reactivity towards mouse and rat IgM

POROS CaptureSelect IgM Affinity Matrix binds to the Fc part of IgM. The high selectivity and yields obtained using IgM Affinity Matrix enable a robust, fast, and efficient purification process with excellent purity obtained in one step.

Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics:

Matrix: Cross-linked poly(styrene-divinylbenzene)
Average particle size: 50 µm
Ligand: CaptureSelect IgM affinity ligand
Ligand coupling method: Carbonyldiimidazole (CDI)
Binding capacity: ∼6 g/L resin (determined with polyclonal human IgM from serum)
Elution conditions: Acidic elution with 0.1 M glycine, pH 3.0
Mechanical resistance: 100 bar (1,450 psi, 10 MPa)
Formulation buffer: 20% (v/v) ethanol

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line
POROS™ CaptureSelect™
Product Type
IgM Affinity Matrix
Particle Size
50 µm
Column Type
Proprietary Resin
Stationary Phase
Anti-IgM
Format
Chromatography Media

Contents & Storage

Store in refrigerator (2–8°C).