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Citations & References (32)
Invitrogen™
ViewRNA™ Tissue Assay Core Kit
The ViewRNA Tissue Assay Core Kit, when combined with a ViewRNA Tissue probe set (purchased separately), enables the detection ofRead more
| Catalog Number | No. of Reactions |
|---|---|
| 19942 | 96 Assays |
Catalog number 19942
Price (HKD)
-
No. of Reactions:
96 Assays
The ViewRNA Tissue Assay Core Kit, when combined with a ViewRNA Tissue probe set (purchased separately), enables the detection of a single mRNA target in tissue sections using the Fast Red substrate. The ViewRNA Tissue Assay Blue Module enables the detection of a second mRNA target using the Fast Blue substrate and must be used in combination with the Core Kit and two probes sets in order to run a 2-plex assay. (Detect up to four RNA or microRNA in cryo or FFPE sectioned tissue with ViewRNA Tissue Fluorescence Assay.) Each kit contains sufficient reagents to process a minimum of six slides at a time. ViewRNA TYPE 1 probe sets are for use with the Fast Red substrate (Core Kit), while ViewRNA TYPE 6 probe sets are for use with the Fast Blue substrate (Blue Module).
ViewRNA Tissue assays are RNA in situ hybridization (ISH) assays for the reliable detection of one or two mRNA targets within tissue sections. Visualize any gene in any tissue with single-copy sensitivity and no radioactivity. And in case your needs are not met by one of our 6500 catalog probe sets, we have decades of bioinformatics expertise and will design custom probe sets to any mRNA target of interest, free of charge.
With ViewRNA Tissue assays, you can achieve:
- Robust, single mRNA molecule detection in tissue sections
- Accuracy and specificity inherent to branched DNA amplification technologies
- Simultaneous detection of two RNA targets
The ViewRNA Tissue Assay is compatible with formalin-fixed, paraffin-embedded (FFPE) tissue. Whereas traditional ISH technologies often amplify both signal and background, ViewRNA assays use branched DNA (bDNA) technology to amplify target-specific signal and achieve superior signal-to-noise ratios.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
No. of Reactions96 Assays
Product LineViewRNA
Product TypeRNA In Situ Hybridization Tissue Assay Core Kit
Quantity96 Assays
Shipping ConditionWet Ice
FormatKit
Unit Size96 assays
Contents & Storage
• 4 x 21 mL 100 x Pretreatment Solution (2°C to 8°C), also available separately (Cat. No. QVT0500)
• 4 x 12 mL Probe Set Diluent (2°C to 8°C), also available separately (Cat. No. QVT0510)
• 4 x 10 mL Pre-Amplifier Mix (2°C to 8°C)
• 4 x 10 mL Amplifier Mix (2°C to 8°C)
• 4 x 24 mL AP (Alkaline Phosphatase) Label Probe Diluent (2°C to 8°C), also available separately (Cat. No. QVT0513)
• 4 x 10 mL AP (Alkaline Phosphatase) Enhancer Solution (2°C to 8°C), also available separately (Cat. No. QVT0508)
• 4 x 20 mL ViewRNA Fast Red Reaction Buffer (2°C to 8°C)
• 4 x 320 μL ViewRNA Fast Red Substrate 1°C(2 to 8°C)
• 4 x 320 μL ViewRNA Fast Red Substrate 2°C(2 to 8°C)
• 4 x 320 μL ViewRNA Fast Red Substrate 3°C(2 to 8°C)
• 4 x 180 μL 100 x Protease Solution (2°C to 8°C), also available separately (Cat. No. QVT0512)
• 4 x 45 μL AP (Alkaline Phosphatase) Label Probe Type 1°C(2 to 8°C)
• 4 x 144 mL 100 x Wash Buffer Component 1°C(15 to 30°C)
• 4 x 280 mL 400 x Wash Buffer Component 2°C(15 to 30°C)
• 4 x 12 mL Probe Set Diluent (2°C to 8°C), also available separately (Cat. No. QVT0510)
• 4 x 10 mL Pre-Amplifier Mix (2°C to 8°C)
• 4 x 10 mL Amplifier Mix (2°C to 8°C)
• 4 x 24 mL AP (Alkaline Phosphatase) Label Probe Diluent (2°C to 8°C), also available separately (Cat. No. QVT0513)
• 4 x 10 mL AP (Alkaline Phosphatase) Enhancer Solution (2°C to 8°C), also available separately (Cat. No. QVT0508)
• 4 x 20 mL ViewRNA Fast Red Reaction Buffer (2°C to 8°C)
• 4 x 320 μL ViewRNA Fast Red Substrate 1°C(2 to 8°C)
• 4 x 320 μL ViewRNA Fast Red Substrate 2°C(2 to 8°C)
• 4 x 320 μL ViewRNA Fast Red Substrate 3°C(2 to 8°C)
• 4 x 180 μL 100 x Protease Solution (2°C to 8°C), also available separately (Cat. No. QVT0512)
• 4 x 45 μL AP (Alkaline Phosphatase) Label Probe Type 1°C(2 to 8°C)
• 4 x 144 mL 100 x Wash Buffer Component 1°C(15 to 30°C)
• 4 x 280 mL 400 x Wash Buffer Component 2°C(15 to 30°C)
Frequently asked questions (FAQs)
Are there any changes in the reagents and buffers used in the ViewRNA Tissue Assay Core Kit relative to the discontinued ViewRNA ISH Tissue Assay kits?
Can I purchase the individual components of the ViewRNA Tissue Assay Core Kit and ViewRNA Tissue Assay Blue Module as standalone products?
How is ViewRNA Tissue Assay Core Kit different from the discontinued ViewRNA ISH Tissue Assay kits?
Label Probe Diluent is provided in the ViewRNA Tissue Assay Core Kit but not in the ViewRNA Tissue Assay Blue Module. Can I purchase this separately?
Citations & References (32)
Citations & References
Abstract
Excessive cytokine response to rapid proliferation of highly pathogenic avian influenza viruses leads to fatal systemic capillary leakage in chickens.
Journal:PLoS One
PubMed ID:23874602
Highly pathogenic avian influenza viruses (HPAIVs) cause lethal infection in chickens. Severe cases of HPAIV infections have been also reported in mammals, including humans. In both mammals and birds, the relationship between host cytokine response to the infection with HPAIVs and lethal outcome has not been well understood. In the
Distribution of serotonin receptor 5-HT6 mRNA in rat neuronal subpopulations: A double in situ hybridization study.
Journal:
PubMed ID:26424380
'The 5-HT6 receptor (5-HT6R) is almost exclusively expressed in the brain and has emerged as a promising target for cognitive disorders, including Alzheimer''s disease. In the present study, we have determined the cell types on which the 5-HT6R is expressed by colocalizing 5-HT6R mRNA with that of a range of
Chemokine (C-C motif) receptor 5 is an important pathological regulator in the development and maintenance of neuropathic pain.
Journal:
PubMed ID:24589480
'The chemokine family has been revealed to be involved in the pathogenesis of neuropathic pain. In this study, the authors investigated the role of chemokine (C-C motif) ligand 3 and its receptors chemokine (C-C motif) receptor 1 and chemokine (C-C motif) receptor (CCR) 5 in neuropathic pain.'
Metabolic regulator LKB1 is crucial for Schwann cell-mediated axon maintenance.
Journal:
PubMed ID:25195104
Schwann cells (SCs) promote axonal integrity independently of myelination by poorly understood mechanisms. Current models suggest that SC metabolism is critical for this support function and that SC metabolic deficits may lead to axonal demise. The LKB1-AMP-activated protein kinase (AMPK) kinase pathway targets several downstream effectors, including mammalian target of
Characterization of target mRNA reduction through in situ RNA hybridization in multiple organ systems following systemic antisense treatment in animals.
Journal:
PubMed ID:24161045
Advances in the medicinal chemistry of antisense oligonucleotide drugs have been instrumental in achieving and optimizing antisense activity in cell types other than hepatocytes, the cell type that is most sensitive to antisense effects following systemic treatment. To broadly characterize the effects of antisense drugs on target messenger RNA (mRNA)