MEM Rega-3 This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  19993013

Related applications:

Mammalian Cell Culture

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Minimum Essential Medium (MEM) REGA-3 is a modification of the commonly used MEM. The Rega-3 modification is often used to culture HEP-G2 (human liver carcinoma) cells and occasionally other human tumor cells. We offer a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With Without
• Phenol Red • L-glutamine
• Gentamycin Sulfate • HEPES
• Sodium Bicarbonate

The complete formulation is available.

Gibco® MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including this Rega-3 modification. MEM Rega-3 is unique because it has a reduced sodium bicarbonate concentration, modified salt concentrations, and contains the antibiotic gentamycin sulfate. This product is made with Earle’s salts.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco® MEM is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

MEM Rega-3 contains no proteins, lipids, or growth factors. Therefore, MEM Rega-3 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (0.85 g ⁄ L) and therefore requires a low CO2 environment to maintain physiological pH.
For Research Use Only. Not for use in diagnostic procedures.


Form: Liquid
Glutamine: No Glutamine
Green Features: Sustainable packaging
Included Antibiotics: Gentamicin
Product Size: 500 mL
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Media formulations