Minimum Essential Medium (MEM) REGA-3 is a modification of the commonly used MEM. The Rega-3 modification is often used to culture HEP-G2 (human liver carcinoma) cells and occasionally other human tumor cells. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.

Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including this Rega-3 modification. MEM Rega-3 is unique because it has a reduced sodium bicarbonate concentration, modified salt concentrations, and contains the antibiotic gentamycin sulfate. This product is made with Earle’s salts.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco™ MEM is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

MEM Rega-3 contains no proteins, lipids, or growth factors. Therefore, MEM Rega-3 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (0.85 g ⁄ L) and therefore requires a low CO₂ environment to maintain physiological pH.