Contents & Storage
Sufficient For: 20 labeling reactions (each with 50 pmol RNA)
• T4 RNA Ligase Reaction Buffer (10X), 100 μL
• T4 RNA Ligase (20,000 U/mL), 40 μL
• Non-labeled RNA Control (10 μM), 100 μL
• Biotinylated IRE RNA Control (125 nM), 35 μL
• RNase Inhibitor (40 U/μL), 2 × 10 μL
• Nuclease-free Water, 1.5 mL
• DMSO, 200 μL
• 30% PEG, 300 μL
• Biotinylated Cytidine (Bis)phosphate (1mM), 40 μL
• Glycogen (20 mg/mL), 20 μL
Store all components at -20°C