Thermo Scientific Pierce Iminobiotin Agarose provides for unusual affinity purification experiments involving clean-up or removal of streptavidin or avidin protein components.
Features of Iminobiotin Agarose:
• Enable purification or removal of avidin or streptavidin samples • Crosslinked beaded agarose onto which iminobiotin is covalently immobilized • Use Iminobiotin Agarose for reversible binding (e.g., non-denaturing purification of avidin)
Pierce Iminobiotin Agarose is beaded agarose support onto which iminobiotin moieties have covalently immobilized. Typical avidin-biotin affinity procedures use immobilized avidin or streptavidin proteins to capture biotin-tagged molecules from a sample. Occasionally, however, the reverse situation arises in which immobilized biotin is needed to capture avidin or streptavidin components from a sample. This iminobiotin resin addresses this special situation.
Immobilized iminobiotin is the guanido analog of biotin. The dissociation constant of the avidin-iminobiotin complex is pH dependent. At pH 9.5 to 11, the avidin-iminobiotin complex will bind tightly. At pH 4, the avidin-iminobiotin complex will dissociate. Because no denaturing agents such as 8M guanidine hydrochloride or 4M urea are required for elution, the avidin conjugate has a better chance of maintaining its activity after purification. Thus, iminobiotin agarose can be used for situations requiring reversible binding and elution of avidin components.
For Research Use Only. Not for use in diagnostic procedures.