Thermo Scientific™

Pierce™ Coomassie Brilliant Blue Dyes

Catalog number: 20278
Thermo Scientific™

Pierce™ Coomassie Brilliant Blue Dyes

Catalog number: 20278
Thermo Scientific Pierce Coomassie Brilliant Blue dyes are composed of one of the most common forms of coomassie dye, which is a key component of various colorimetric protein gel stains. Coomassie R-250 and G-250 dyes are two chemical forms of a disulfonated triphenylmethane compound that is commonly used as the basis of stains for detection of proteins in gel electrophoresis and Bradford-type assay reagents for protein quantitation. The R-250 (red-tinted) form lacks two methyl groups that are present in the G-250 (green-tinted) form, which is also called colloidal coomassie dye.
 
Catalog Number
20278
Unit Size
50 g
Description
Pierce Coomassie Brilliant Blue R-250 Dye
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionPierce Coomassie Brilliant Blue R-250 Dye
Detection LocationIn-Gel Detection, In-Solution Detection, In-Blot Detection
Detection MethodColorimetric
Label or DyeCoomassie
Product TypeProtein Gel Stain
Quantity50 g
Target MoleculeProtein
Unit Size50 g
Catalog Number
Unit Size
50 g
Description
Pierce Coomassie Brilliant Blue G-250 Dye
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionPierce Coomassie Brilliant Blue G-250 Dye
Detection LocationIn-Gel Detection, In-Solution Detection, In-Blot Detection
Detection MethodColorimetric
Label or DyeCoomassie
Product TypeProtein Gel Stain
Quantity50 g
Target MoleculeProtein
Unit Size50 g
Showing 2 of 2
Catalog NumberSpecificationsUnit SizeDescriptionPrice (USD)AvailabilityQuantity
20278Full specifications
50 gPierce Coomassie Brilliant Blue R-250 Dye
-
DescriptionPierce Coomassie Brilliant Blue R-250 Dye
Detection LocationIn-Gel Detection, In-Solution Detection, In-Blot Detection
Detection MethodColorimetric
Label or DyeCoomassie
Product TypeProtein Gel Stain
Quantity50 g
Target MoleculeProtein
Unit Size50 g
20279Full specifications
50 gPierce Coomassie Brilliant Blue G-250 Dye
-
DescriptionPierce Coomassie Brilliant Blue G-250 Dye
Detection LocationIn-Gel Detection, In-Solution Detection, In-Blot Detection
Detection MethodColorimetric
Label or DyeCoomassie
Product TypeProtein Gel Stain
Quantity50 g
Target MoleculeProtein
Unit Size50 g
Showing 2 of 2
Coomassie R-250 and G-250 dyes are two chemical forms of a disulfonated triphenylmethane compound that is commonly used as the basis of stains for detection of proteins in gel electrophoresis and Bradford-type assay reagents for protein quantitation. The R-250 (red-tinted) form lacks two methyl groups that are present in the G-250 (green-tinted) form, which is also called colloidal coomassie dye.

Typically, coomassie gel stains and protein assay reagents are formulated as very acidic solutions in 25 to 50% methanol. In acidic conditions, the dye binds to proteins primarily through basic amino acids (primarily arginine, lysine, and histidine), and the number of coomassie dye ligands bound to each protein molecule is approximately proportional to the number of positive charges found on the protein. Protein binding causes the dye to change from reddish-brown to bright blue (absorption maximum equals 595 nm).

Features include:
Easy detection—develops intensely colored complexes with proteins
High sensitivity—can determine as little as 0.5 μg/cm2 of protein present in a gel matrix
Reversible staining—anion of Coomassie Brilliant Blue dye formed in the acidic staining medium combines with the protonated amino groups of proteins by electrostatic interaction; resulting complex is reversible under proper conditions
Differentiation between bound and unbound dye—when dissolved in 0.01 M citrate buffer at pH 3.0, has an absorption maximum at 555 nm; protein-dye complex is characterized by a peak slightly broader than that of free dye with a maximum at 549 nm

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Store at room temperature.

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