Pierce™ DTT (ditiotreitol)
Pierce™ DTT (ditiotreitol)
Thermo Scientific™

Pierce™ DTT (ditiotreitol)

El ditiotreitol es un agente reductor ampliamente utilizado en muchas aplicaciones de investigación de proteínas, incluida la electroforesis de poliacrilamidaMás información
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Número de catálogoCantidad
202905 g
Número de catálogo 20290
Precio (MXN)
-
Cantidad:
5 g
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El ditiotreitol es un agente reductor ampliamente utilizado en muchas aplicaciones de investigación de proteínas, incluida la electroforesis de poliacrilamida reductora (SDS-PAGE).

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Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
DescripciónPierce DTT, reactivo de Cleland
FormularioPolvo
Línea de productosPierce
Cantidad5 g
Tipo de productoDitiotreitol
Unit SizeEach
Contenido y almacenamiento
Almacenar a 4 °C.

Preguntas frecuentes

How do I prepare a 1 M DTT solution from powder and how should I store the solution?

Preparing 1 M DTT:
1) Dissolve 3.09 g DTT in 20 mL distilled H2O.
2) Dispense into 1 mL aliquots.
3) Store the aliquots at -20 degrees C.
Note: Upon thawing the vial, there may be some precipitation that can be re-dissolved by warming and mixing.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can you please recommend a method for reduction, alkylation, and digestion of an antibody?

Prepare 1 mg/mL antibody in 7.0 M Guanidine HCl, 100 mM Tris, pH 8.3. For reduction, add DTT (Cat. Nos. A39255, 20290) solution at 10 mM final concentration and incubate for 30 mins at room temperature. For alkylation, add sodium iodoacetate at 20 mM final concentration and incubate in the dark for 20 mins at room temperature. Perform buffer exchange followed by digestion using Pierce MS grade Trypsin, Cat. Nos. 90058, 90057 (1:10 enzyme to substrate ratio).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.