SulfoLink™ Immobilization Trial Kit, 2 mL

Catalog number: 20325

Thermo Scientific™  Related applications: Protein Purification & Isolation

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Description

Thermo Scientific SulfoLink Immobilization Trial Kit, 2 mL is a single-column trial kit available for use with either peptides or proteins.

Features of SulfoLink Immobilization Trial Kit, 2 mL:

Specific to sulfhydryl (-SH) groups—iodoacetyl groups react specifically with sulhydryls to form irreversible thioether bonds
Fast—spin columns increase protocol speed; prepare and couple samples in 2 hours (peptides) to 3.5 hours (proteins)
Flexible coupling conditions—use pH 7.5 to 9.0 aqueous buffers, organic solvent (e.g., 20% DMSO) or denaturant (guanidine·HCl), as needed for protein or peptide solubility during coupling reaction
Easy-to-follow instructions—streamlined protocols for sample preparation, immobilization, and affinity purification
High capacity—Immobilize 1 to 2 mg of peptide or 2 to 20 mg of protein per 2 mL column of SulfoLink Coupling Resin

Applications:
• Immobilize peptides having terminal cysteine residues to purify antibodies that were generated against peptide immunogens prepared by maleimide conjugation
• Immobilize antibodies in oriented manner through hinge-region sulfhydryls to ensure that antigen binding sites are not sterically hindered for antigen affinity purification

When incubated with a solution of peptide or protein that contains reduced cysteine residues, the iodoacetyl groups of SulfoLink Resin react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the peptide or protein to the resin. The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest. Choose one of two convenient SulfoLink Immobilization Kits for peptides or proteins. Each kit contains all the reagents for preparing the sample and five columns containing 2 mL of SulfoLink Resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification.

Related Products
SulfoLink™ Coupling Resin
SulfoLink™ Immobilization Kit for Peptides, 2 mL
SulfoLink™ Immobilization Kit for Proteins, 2 mL
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Spin Column, Kit, 15 mL conical
Column Type: Affinity, Agarose Resin
Product Size: 1 column
Immobilized Phase: Haloacetyl

Contents & storage

Sufficient For: Immobilizing 1 mg of peptide or 10 mg protein per column
• SulfoLink Column, 2 mL, 1 column
• SulfoLink Preparation Buffer, 7.5 mL
• SulfoLink Coupling Buffer, 120 mL
• Wash Solution, 25 mL
• PBS Pack (makes 500 mL), 1 pack
• 2-Mercaptoethylamine·HCl, 6 mg
• Bond-Breaker TCEP Solution, Neutral pH, 0.5 mL
• L-Cysteine·HCl, 100 mg
• Zeba Spin Desalting Column, 5 mL, 1 column
• Column Accessories

Upon receipt, store components at 4°C protected from light.

Documents

Manuals & protocols

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