UltraLink™ Iodoacetyl Micro Peptide Coupling Kit, 0.1 mL

Catalog number:  20485

Thermo Scientific™  Related applications: Protein Purification & Isolation

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The Thermo Scientific UltraLink Iodoacetyl Micro Peptide Coupling Kit enables covalent immobilization of cysteine-containing peptides for use in antibody purification and other affinity protocols.

Features of the Micro Peptide Coupling Kit:

Optimized for small scale—ideal for coupling small amounts (25 to 250 µg) of sulfhydryl-containing peptide or protein for purification of specific antibodies from crude serum
High-performance affinity resin—uses durable, polyacrylamide-based UltraLink Iodoacetyl Resin for specific reaction to sulfhydryl groups
Efficient coupling chemistry—immobilization efficiency greater than 85% (as measured with 1 hour reaction using insulin, calcitonin and osteocalcin peptides)
Fast and easy to use—perform wash and elution steps using a microcentrifuge
Reusable—use prepared peptide resin several times with no significant loss of capacity

The Micro Peptide Kit is for immobilizing small amounts (25 to 250 µg) of sulfhydryl-containing peptides (e.g., cysteine-terminated peptides) onto a beaded porous resin to create a small, reusable, microcentrifuge affinity column. The coupling and affinity purification procedures are optimized for small sample volumes (200 to 300 µL). Wash and elution steps are achieved rapidly and efficiently with the convenient microcentrifuge spin columns. Each kit contains sufficient reagents for 10 coupling reactions and 20 affinity purifications. The kit is ideal for immobilizing peptide antigens that contain a terminal cysteine residue for use in purifying specific antibodies from small serum, ascites or culture supernatant samples.

Related Products
UltraLink™ Iodoacetyl Resin
For Research Use Only. Not for use in diagnostic procedures.


Column Type: Affinity, UltraLink™ Resin
Format: Kit, Microcentrifuge, Spin Column
Immobilized Phase: Haloacetyl
Product Line: UltraLink™
Product Size: 10 columns

Contents & storage

Sufficient For: Immobilizing 25–250 µg of peptide or protein per microcentrifuge column; Includes buffers for 20 affinity purifications
• UltraLink Iodoacetyl Spin Columns, 0.1 mL, 10 columns
• Coupling Buffer, 100 mL
• L-Cysteine·HCl, 100 mg
• Wash Solution, 25 mL
• PBS Pack (makes 500 mL), 1 pack
• IgG Elution Buffer, 50 mL
• Collection Tubes, 200 tubes
Store at 4°C protected from light.


Manuals & protocols