Thermo Scientific™

Pierce™ Protein A IgG Binding Buffer

Catalog number:  21007

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Thermo Scientific Pierce IgG Binding and Elution Buffers ensure high-yield, nondenaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads. The Protein A IgG Binding Buffer is a phosphate-based buffer containing glycerol and EDTA, formulated for antibody purification using Protein A.

Shared features of all Thermo Scientific Pierce IgG Binding and Elution Buffers:

Ready to use—carefully formulated and prefiltered 1X solutions
Three binding buffers—optimized for Protein A, Protein G or Protein A/G
Two elution buffer options—acidic (pH 2.8) or high salt (near-neutral)
Versatile—elution buffers are appropriate for antibody-antigen affinity purification

Products available for each antibody purification method:
Protein A IgG Binding Buffer: pH 8.0; phosphate-based; contains glycerol and EDTA
Protein A/G IgG Binding Buffer: pH 8.0; phosphate-based; contains EDTA
Protein G IgG Binding Buffer: pH 5.0; contains 0.02% sodium azide
IgG Elution Buffer: pH 2.8; amine-based
Gentle Ag/Ab Binding Buffer: pH 8.0; phosphate-free; contains 0.05% sodium azide
Gentle Ag/Ab Elution Buffer: pH 6.6; high salt (ionic strength)

Related Products
Pierce™ IgG Elution Buffer
Pierce™ Protein G IgG Binding Buffer
For Research Use Only. Not for use in diagnostic procedures.


Form: Liquid
Product Line: Pierce™
Product Size: 3.75 L
Reagent Type: Binding Buffer

Contents & storage

Store at 4°C.


Manuals & protocols