Basal Medium Eagle (BME)
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Gibco™
Basal Medium Eagle (BME)
Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells.Read more
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Catalog number 21010046
Price (USD)/ 500 mL
34.65
Online exclusive
37.74 
Save 3.09 (8%)
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Price (USD)/ 500 mL
34.65
Online exclusive
37.74 
Save 3.09 (8%)
Add to cart
Basal Medium Eagle (BME)
Catalog number21010046
Price (USD)/ 500 mL
34.65
Online exclusive
37.74 
Save 3.09 (8%)
-
Add to cart

Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells. BME was originally developed by Harry Eagle for HeLa cells and mouse fibroblasts, when he discovered the minimum requirements for cell growth in vitro. BME has since been used for other human lines, including WI-38 and MRC-5.

This BME is manufactured as follows:

  • With: Phenol Red
  • Without: L-glutamine, HEPES

The complete formulation is available.

BME contains eight B vitamins, the ten essential amino acids, plus cystine, tyrosine, and glutamine. There are a number of modifications of BME: Minimal Essential Medium, Glasgow's Medium and Dulbecco's Modified Eagle's Medium. This BME formulation contains Earle's salts for use in a CO2 incubator.

BME contains no proteins, lipids, or growth factors. Therefore, BME requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). BME uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineHeLa, mouse fibroblasts, WI-38 and MRC-5
ClassificationAnimal Origin-free
Concentration1 X
FormLiquid
Product TypeBasal Medium Eagle (BME)
Shelf Life12 Months From Date of Manufacture
SterilitySterile-filtered
With AdditivesLow Glucose, Phenol Red
Without AdditivesNo Glutamine, No HEPES, No Sodium Pyruvate
Green FeaturesSustainable packaging
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Quantity500 mL
Shipping ConditionRoom Temperature
Unit Size500 mL
Contents & Storage
Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture