MEM, glutamine, HEPES, powder

Catalog number: 21011044

Gibco™  Related applications: Mammalian Cell Culture

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Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With Without
• L-glutamine • Sodium Bicarbonate
• Phenol Red

The complete formulation is available.

Gibco® MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco® MEM is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Powder forms of Gibco® L-15 cell culture medium require pH adjustment and filtration at the time of preparation (see protocol for details).
For Research Use Only. Not for use in diagnostic procedures.


Glutamine: L-Glutamine
Phenol Red Indicator: Phenol Red
Form: Powder
Sodium Pyruvate Additive: No Sodium Pyruvate
pH Range: 5.2 - 5.6
Osmolality: 240 - 280 mOsm/kg
Product Size: 50 L
Classification: Animal Origin-Free
Culture Environment: CO2
Serum Supplementation: Standard Serum Supplementation
Supplementation Required: Sodium Bicarbonate, Serum
Sodium Bicarbonate Buffer: No Sodium Bicarbonate
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8° C.
Shipping conditions: Ambient.
Shelf life: 36 months from date of manufacture.


Manuals & protocols

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