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Pierce™ Western Blot Signal Enhancer - FAQs

View additional product information for Pierce™ Western Blot Signal Enhancer - FAQs (21050)

9 product FAQs found

How can I increase detection sensitivity with SuperSignal West Femto Maximum Sensitivity Substrate?

Treating the nitrocellulose or PVDF membrane with Western Blot Signal Enhancer (Cat. No. 21050) before blocking can boost the chemiluminescent signal of low-intensity bands.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How can I increase detection sensitivity with SuperSignal West Dura Extended Duration Substrate?

Treating the nitrocellulose or PVDF membrane with Western Blot Signal Enhancer (Cat. No. 21050) before blocking can boost the chemiluminescent signal of low-intensity bands.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the difference between Western Blot Signal Enhancer and SuperSignal Western Blot Enhancer? Is there any benefit to one versus the other?

The reagents in both kits are different. Western Blot Signal Enhancer (Cat. No. 21050) is a 2-step pre-treatment that occurs before blocking. It basically relaxes the protein on the membrane making the epitope easier to detect. SuperSignal Western Blot Enhancer (Cat. Nos. 46640, 46641) contains a membrane treatment reagent and a primary antibody diluent that increase both signal intensity and sensitivity 3- to 10-fold compared to a detecion performed without it. We have found that SuperSignal Western Blot Enhancer shows much less background while enhancing signal and sensitivity when compared to Western Blot Signal Enhancer.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Will the Pierce Western Blot Signal Enhancer interfere with protein detection?

The Enhancer does not bind to the membrane or proteins so it should not interfere with an antibody in binding the target protein.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I use the Pierce Western Blot Signal Enhancer on a stripped blot?

After stripping the blot, blocking reagent remains on the membrane so you will get inconsistent results. Therefore, we recommend using the Enhancer only before blocking and the first probing of the membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I stain my membrane with Ponceau S prior to using the Pierce Western Blot Signal Enhancer?

We recommend using the Enhancer prior to staining and blocking.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do I need to use a Pierce western blotting substrate with the Pierce Western Blot Signal Enhancer?

The Enhancer will work for any chemiluminescent or colorimetric substrate.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How does the Pierce Western Blot Signal Enhancer make the signal stronger?

The Enhancer relaxes the proteins, allowing the antibody to bind to the protein more efficiently.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

When should I use the Pierce Western Blot Signal Enhancer?

The Pierce Western Blot Signal Enhancer should be used after transferring proteins to the membrane and before blocking or staining the membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.