Informational:Informational:Create physiologically relevant cell culture models with Gibco Human Plasma-like Medium >
Gibco™

DMEM, high glucose, HEPES, no phenol red

Green features
Catalog number: 21063029
Gibco™

DMEM, high glucose, HEPES, no phenol red

Green features
Catalog number: 21063029
Catalog Number
21063029
also known as 21063-029
Unit Size
500 mL
Price (USD)
67.00
Your Price:
Availability
***
Quantity
Catalog Number
21063045
also known as 21063-045
Unit Size
10 x 500 mL
Price (USD)
588.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
21063029
also known as 21063-029
500 mL
67.00
Your Price:
***
21063045
also known as 21063-045
10 x 500 mL
588.00
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
Recommendations
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:
WithWithout
• High Glucose• Sodium Pyruvate
• L-glutamine• Phenol Red
• HEPES

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For In Vitro Diagnostic Use. For Research Use Only in China.

Specifications

Concentration
1X
Product Type
DMEM (Dulbecco's Modified Eagle Medium)
Sterility
Sterile-filtered
Glucose Concentration
4500 mg/L
Serum Supplementation
Standard Serum Supplementation
Shipping Condition
Room Temperature
Volume (Metric)
500 ml
Product Line
Gibco™
Form
Liquid
Glutamine
L-Glutamine
Phenol Red Indicator
No Phenol Red
Shelf Life
12 Months From Date of Manufacture
Green Features
Sustainable packaging
Quantity
500mL
Cell Type
primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells
With Additives
High Glucose, L-Glutamine, HEPES
Without Additives
Sodium Pyruvate, Phenol Red
Glucose
High Glucose
Sodium Pyruvate Additive
No Sodium Pyruvate
Cell Line
HeLa, 293, Cos-7, and PC-12
Classification
Animal Origin-Free
Solution Type
DMEM (Dulbecco's Modified Eagle Medium)
HEPES Buffer
HEPES

Contents & Storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Figures