Medium 199, Earle's Salts (10X) This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: 21180021

Gibco™  Related applications: Mammalian Cell Culture

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Description

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Medium 199 is modified as follows:

WithWithout
• Phenol Red• HEPES
• L-glutamine• Sodium Bicarbonate

The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco® Medium 199 product made in our Grand Island facility (11825-015). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Concentrated forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Specifications

Glutamine: L-Glutamine
Phenol Red Indicator: Phenol Red
Form: Liquid
HEPES Buffer: No HEPES
Sodium Pyruvate Additive: Sodium Pyruvate
pH Range: 2.0 - 2.8
Osmolality: 2400 - 2900 mOsm/kg
Concentrated: 10 X
Product Size: 500 mL
Classification: Animal Origin
Volume (Metric): 500 ml
Culture Environment: CO2
Supplementation Required: Sodium Bicarbonate, Serum
Sodium Bicarbonate Buffer: No Sodium Bicarbonate
Green Features: Fewer resources used, Less waste
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.

Media formulations

Documents

Manuals & protocols

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