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View additional product information for EZ-Link™ Sulfo-NHS-LC-Biotin, No-Weigh™ Format - FAQs (21327)
5 product FAQs found
No. NHS esters hydrolyze very quickly. The container must be brought to room temperature (RT) prior to opening, then the amount of biotinylation reagent weighed, dissolved and used immediately.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Yes. Because this molecule dissolves readily in polar solutions and is itself charged by the sodium sulfoxide group on the succinimidyl ring, it cannot permeate the cell wall or membrane of an intact cell. As long as the cell is intact, only the primary amines on the surface will be biotinylated.
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Tris is a primary amine, which will directly compete with protein primary amines for reaction with the NHS-ester activated biotin reagent. However, once a protein is labeled and desalted, Tris buffer can be used for storage or assay buffers, as appropriate for the protein and application.
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The molar excess to use depends on the concentration of the ligand to be biotinylated. Although the literature contains references that use between 2-50 molar excess of biotin, we recommend the following: 10-fold molar excess for 10 mg/mL solution of protein; 25-fold molar excess for 2 mg/mL solution of protein.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
EZ-Link Sulfo-NHS-LC-Biotin has a spacer arm length of 22.4 Angstroms while EZ-Link Sulfo-NHS-LC-LC-Biotin has a spacer arm length of 30.5 Angstroms.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.