Get out of the cold & warm up to new Gibco BenchStable Media! Optimized for storage at room temperature »
Gibco™

DMEM/F-12, no glutamine This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  21331020

Related applications:

Mammalian Cell Culture

Back to top

Description

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco® DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM/F-12 is modified as follows:

WithWithout
Phenol RedL-glutamine
HEPES
The complete formulation is available.

Gibco® DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids and vitamins with F-12's wide variety of components.

Product Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® DMEM/F-12 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Specifications

HEPES Buffer: No HEPES
Serum Supplementation: Standard Serum Supplementation
Shipping Condition: Room Temperature
Volume (Metric): 500 ml
Product Line: Gibco™
Form: Liquid
Glutamine: No Glutamine
Phenol Red Indicator: Phenol Red
Green Features: Sustainable packaging
Quantity: 500mL
Glucose: High Glucose
Sodium Pyruvate Additive: Sodium Pyruvate

Contents & storage

Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture