Automated screening for small organic ligands using DNA-encoded chemical libraries.
AuthorsDecurtins W, Wichert M, Franzini RM, Buller F, Stravs MA, Zhang Y, Neri D, Scheuermann J
JournalNat Protoc
PubMed ID26985574
'DNA-encoded chemical libraries (DECLs) are collections of organic compounds that are individually linked to different oligonucleotides, serving as amplifiable identification barcodes. As all compounds in the library can be identified by their DNA tags, they can be mixed and used in affinity-capture experiments on target proteins of interest. In this ... More
Lower allergen levels in hypoallergenic Curly Horses? A comparison among breeds by measurements of horse allergens in hair and air samples.
AuthorsZahradnik E, Janssen-Weets B, Sander I, Kendzia B, Mitlehner W, May C, Raulf M
JournalPLoS One
PubMed ID30540798
'Exposure to horses can cause severe allergic reactions in sensitized individuals. The breed, American Bashkir Curly Horse is categorized as hypoallergenic, primarily due to reports of allergic patients experiencing fewer symptoms while handling this special breed. The possible reasons for this phenomenon could be lower allergen production and/or reduced allergen ... More
Clinical on-site monitoring of ß-lactam antibiotics for a personalized antibiotherapy.
AuthorsBruch R, Chatelle C, Kling A, Rebmann B, Wirth S, Schumann S, Weber W, Dincer C, Urban G
JournalSci Rep
PubMed ID28600499
An appropriate antibiotherapy is crucial for the safety and recovery of patients. Depending on the clinical conditions of patients, the required dose to effectively eradicate an infection may vary. An inadequate dosing not only reduces the efficacy of the antibiotic, but also promotes the emergence of antimicrobial resistances. Therefore, a ... More
Cross-talk between miR-471-5p and autophagy component proteins regulates LC3-associated phagocytosis (LAP) of apoptotic germ cells.
AuthorsPanneerdoss S, Viswanadhapalli S, Abdelfattah N, Onyeagucha BC, Timilsina S, Mohammad TA, Chen Y, Drake M, Vuori K, Kumar TR, Rao MK
JournalNat Commun
PubMed ID28928467
Phagocytic clearance of apoptotic germ cells by Sertoli cells is vital for germ cell development and differentiation. Here, using a tissue-specific miRNA transgenic mouse model, we show that interaction between miR-471-5p and autophagy member proteins regulates clearance of apoptotic germ cells via LC3-associated phagocytosis (LAP). Transgenic mice expressing miR-471-5p in ... More
IRS-1 acts as an endocytic regulator of IGF-I receptor to facilitate sustained IGF signaling.
AuthorsYoneyama Y, Lanzerstorfer P, Niwa H, Umehara T, Shibano T, Yokoyama S, Chida K, Weghuber J, Hakuno F, Takahashi SI
JournalElife
PubMed ID29661273
Insulin-like growth factor-I receptor (IGF-IR) preferentially regulates the long-term IGF activities including growth and metabolism. Kinetics of ligand-dependent IGF-IR endocytosis determines how IGF induces such downstream signaling outputs. Here, we find that the insulin receptor substrate (IRS)-1 modulates how long ligand-activated IGF-IR remains at the cell surface before undergoing endocytosis ... More
Non-canonical Ret signaling augments p75-mediated cell death in developing sympathetic neurons.
AuthorsDonnelly CR, Gabreski NA, Suh EB, Chowdhury M, Pierchala BA
JournalJ Cell Biol
PubMed ID30018091
Programmed cell death (PCD) is an evolutionarily conserved process critical in sculpting many organ systems, yet the underlying mechanisms remain poorly understood. Here, we investigated the interactions of pro-survival and pro-apoptotic receptors in PCD using the sympathetic nervous system as a model. We demonstrate that Ret, a receptor tyrosine kinase ... More
Detection of IL-36? through noninvasive tape stripping reliably discriminates psoriasis from atopic eczema.
AuthorsBerekméri A, Latzko A, Alase A, Macleod T, Ainscough JS, Laws P, Goodfield M, Wright A, Helliwell P, Edward S, Brown GD, Reid DM, Wenzel J, Stacey M, Wittmann M
JournalJ Allergy Clin Immunol
PubMed ID29782895
Promotion of homology-directed DNA repair by polyamines.
AuthorsLee CY, Su GC, Huang WY, Ko MY, Yeh HY, Chang GD, Lin SJ, Chi P
JournalNat Commun
PubMed ID30622262
Polyamines, often elevated in cancer cells, have been shown to promote cell growth and proliferation. Whether polyamines regulate other cell functions remains unclear. Here, we explore whether and how polyamines affect genome integrity. When DNA double-strand break (DSB) is induced in hair follicles by ionizing radiation, reduction of cellular polyamines ... More
Studies to identify novel immune-regulatory functions of active vitamin D (1,25(OH)
An array of 60,000 antibodies for proteome-scale antibody generation and target discovery.
AuthorsWang Z, Li Y, Hou B, Pronobis MI, Wang M, Wang Y, Cheng G, Weng W, Wang Y, Tang Y, Xu X, Pan R, Lin F, Wang N, Chen Z, Wang S, Ma LZ, Li Y, Huang D, Jiang L, Wang Z, Zeng W, Zhang Y, Du X, Lin Y, Li Z, Xia Q, Geng J, Dai H, Yu Y, Zhao XD, Yuan Z, Yan J, Nie Q, Zhang X, Wang K, Chen F, Zhang Q, Zhu Y, Zheng S, Poss KD, Tao SC, Meng X
JournalSci Adv
PubMed ID32195335
Antibodies are essential for elucidating gene function. However, affordable technology for proteome-scale antibody generation does not exist. To address this, we developed Proteome Epitope Tag Antibody Library (PETAL) and its array. PETAL consists of 62,208 monoclonal antibodies (mAbs) against 15,199 peptides from diverse proteomes. PETAL harbors binders for a great ... More