EZ-Link™ Sulfo-NHS-LC-LC-Biotin

EZ-Link™ Sulfo-NHS-LC-LC-Biotin - FAQs

View additional product information for EZ-Link™ Sulfo-NHS-LC-LC-Biotin - FAQs (21338B)

3 product FAQs found

What is the difference between EZ-Link Sulfo-NHS-LC-Biotin and Sulfo-NHS-LC-LC-Biotin?

EZ-Link Sulfo-NHS-LC-Biotin has a spacer arm length of 22.4 Angstroms while EZ-Link Sulfo-NHS-LC-LC-Biotin has a spacer arm length of 30.5 Angstroms.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Is there any advantage of choosing a Sulfo-NHS biotin with a longer spacer arm than EZ-Link Sulfo NHS Biotin?

Sometimes. The biotin has to insert 9 angstrom into the pocket of avidin, streptavidin or NeutrAvidin to bind. If steric hindrance may be a problem, using a biotinylation reagent with a longer spacer arm (Sulfo-NHS-LC Biotin or Sulfo-NHS-LC-LC Biotin) may result in greater purification or deteciton.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Why would I chose a Sulfo-NHS Biotin over its non-sulfonated counterpart?

The sodium sulfonate group lends water solubility to the reagent.
Note: The actual biotin moiety that is added is identical between NHS and Sulfo-NHS biotins that vary only in the addition of the sodium sulfonate on the leaving group. Researchers preferring a more water soluble biotin be added to their molecule should consider a PEGylated version of biotin.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.