Medium NCTC-109 (1X) This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  21340039

 Related applications:

Antibody & Cell Culture Production

| Mammalian Cell Culture

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NCTC-109 medium is a chemically defined medium that was originally developed by the National Cancer Institute and formulated for the culture of mouse Strain L sublines, NCTC clone 929 (L929 cells) in a serum-free environment. Gibco® NCTC-109 medium is also suitable for generating and maintaining hybridoma cells.
This NCTC-109 is modified as follows:
• Phenol Red• L-glutamine
• Coenzymes• HEPES

The complete formulation is available.

Gibco® NCTC-109 medium is rich in nucleosides, vitamins, coenzymes, reducing agents and other unique ingredients.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® NCTC-109 medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® NCTC-109 product made in our Scotland facility (21340-054). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

NCTC-109 contains no proteins, lipids, or growth factors. NCTC-109 medium typically requires supplementation of a growth factor cocktail (for serum free growth) or Fetal Bovine Serum (FBS). NCTC-109 medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.


Cell Type: Hybridomas
Form: Liquid
Glucose: Low Glucose
Glutamine: No Glutamine
Phenol Red Indicator: Phenol Red
Product Line: Gibco®
Product Size: 500 mL
Sodium Pyruvate Additive: No Sodium Pyruvate
Volume (Metric): 500 ml
Green Features: Sustainable packaging
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture

Media formulations


Manuals & protocols