Medium NCTC-109 (1X) This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: 21340039

Gibco™  Related applications: Antibody & Cell Culture Production | Mammalian Cell Culture

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


NCTC-109 medium is a chemically defined medium that was originally developed by the National Cancer Institute and formulated for the culture of mouse Strain L sublines, NCTC clone 929 (L929 cells) in a serum-free environment. Gibco® NCTC-109 medium is also suitable for generating and maintaining hybridoma cells.
This NCTC-109 is modified as follows:
• Phenol Red• L-glutamine
• Coenzymes• HEPES

The complete formulation is available.

Gibco® NCTC-109 medium is rich in nucleosides, vitamins, coenzymes, reducing agents and other unique ingredients.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® NCTC-109 medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® NCTC-109 product made in our Scotland facility (21340-054). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

NCTC-109 contains no proteins, lipids, or growth factors. NCTC-109 medium typically requires supplementation of a growth factor cocktail (for serum free growth) or Fetal Bovine Serum (FBS). NCTC-109 medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.


BrazilCouponProductLists: Dealers_40
Form: Liquid
Glucose: Low Glucose
Cell Type: Hybridomas
Glutamine: No Glutamine
Product Size: 500 mL
Volume (Metric): 500 ml
Phenol Red Indicator: Phenol Red
Sodium Pyruvate Additive: No Sodium Pyruvate
Green Features: Fewer resources used, Less waste
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture

Media formulations


Manuals & protocols

Recommended products