Design and characterization of mouse IgG1 and IgG2a bispecific antibodies for use in syngeneic models.
AuthorsWang F, Tsai JC, Davis JH, Chau B, Dong J, West SM, Hogan JM, Wheeler ML, Bee C, Morishige W, Cayton T, David-Brown D, Zhang C, Kozhich A, Sproul T, Dollinger G, Rajpal A, Strop P
Journal
PubMed ID31856660
The development of antibody therapeutics relies on animal models that accurately recapitulate disease biology. Syngeneic mouse models are increasingly used with new molecules to capture the biology of complex cancers and disease states, and to provide insight into the role of the immune system. The establishment of syngeneic mouse models ... More
Ensembles of Bidirectional Kinesin Cin8 Produce Additive Forces in Both Directions of Movement.
AuthorsFallesen T, Roostalu J, Duellberg C, Pruessner G, Surrey T
JournalBiophys J
PubMed ID29117528
Most kinesin motors move in only one direction along microtubules. Members of the kinesin-5 subfamily were initially described as unidirectional plus-end-directed motors and shown to produce piconewton forces. However, some fungal kinesin-5 motors are bidirectional. The force production of a bidirectional kinesin-5 has not yet been measured. Therefore, it remains ... More
Intermittent Hypoxia Activates Duration-Dependent Protective and Injurious Mechanisms in Mouse Lung Endothelial Cells.
AuthorsWohlrab P, Soto-Gonzales L, Benesch T, Winter MP, Lang IM, Markstaller K, Tretter V, Klein KU
JournalFront Physiol
PubMed ID30574096
Intermittent hypoxia is a major factor in clinical conditions like the obstructive sleep apnea syndrome or the cyclic recruitment and derecruitment of atelectasis in acute respiratory distress syndrome and positive pressure mechanical ventilation. ... More
Targeting the Non-catalytic RVxF Site of Protein Phosphatase-1 With Small Molecules for Ebola Virus Inhibition.
AuthorsLin X, Ammosova T, Choy MS, Pietzsch CA, Ivanov A, Ahmad A, Saygideger Y, Kumari N, Kovalskyy D, Üren A, Peti W, Bukreyev A, Nekhai S
JournalFront Microbiol
PubMed ID31572348
Ebola virus (EBOV) is a non-segmented negative-sense RNA virus that causes a severe human disease. The ongoing EBOV outbreak in the Eastern part of Democratic Republic of the Congo has resulted to date in over 2500 confirmed cases including over 1500 deaths. Difficulties with vaccine administration indicate the necessity for ... More
Multiplexed, high-throughput measurements of cell contraction and endothelial barrier function.
AuthorsRokhzan R, Ghosh CC, Schaible N, Notbohm J, Yoshie H, Ehrlicher AJ, Higgins SJ, Zhang R, Haller H, Hardin CC, David S, Parikh SM, Krishnan R
JournalLab Invest
PubMed ID30310180
Vascular leakage, protein exudation, and edema formation are events commonly triggered by inflammation and facilitated by gaps that form between adjacent endothelial cells (ECs) of the vasculature. In such paracellular gap formation, the role of EC contraction is widely implicated, and even therapeutically targeted. However, related measurement approaches remain slow, ... More
Ultrasound-mediated blood-brain barrier opening enhances delivery of therapeutically relevant formats of a tau-specific antibody.
AuthorsJanowicz PW, Leinenga G, Götz J, Nisbet RM
JournalSci Rep
PubMed ID31239479
The microtubule-associated protein tau is an attractive therapeutic target for the treatment of Alzheimer's disease and related tauopathies as its aggregation strongly correlates with disease progression and is considered a key mediator of neuronal toxicity. Delivery of most therapeutics to the brain is, however, inefficient, due to their limited ability ... More
The New Application of UHPLC-DAD-TOF/MS in Identification of Inhibitors on ß-Amyloid Fibrillation From
AuthorsYu L, Wu AG, Wong VK, Qu LQ, Zhang N, Qin DL, Zeng W, Tang B, Wang HM, Wang Q, Law BY
JournalFront Pharmacol
PubMed ID30936829
Literary evidence depicts that aggregated ß-amyloid (Aß) leads to the pathogenesis of Alzheimer's disease (AD). Although many traditional Chinese medicines (TCMs) are effective in treating neurodegenerative diseases, there is no effective way for identifying active compounds from their complicated chemical compositions. Instead of using a traditional herbal separation method with ... More