Thermo Scientific™
Pierce™ GST Protein Interaction Pull-Down Kit
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Description
Thermo Fisher Scientific Pierce GST Tag Protein Interaction Pull-Down Kits contains the necessary components to capture and purify proteins that interact with GST-tagged fusion proteins.Features of GST Tag Protein Interaction Pull-Down Kit:
• GST pull-down (Product No. 21516)—gently purifies protein interactors of any GST-tagged fusion protein without denaturing
• Complete kits—provide all components and detailed protocol for purifying protein:protein interactions
• No special equipment needed—use common laboratory equipment and reagents (e.g., microcentrifuge)
• Convenient—microcentrifuge spin columns facilitate simple and efficient manipulation of agarose beads, including simple processing of multiple samples
• Flexible—instructions include protocols for use with bait and prey proteins from many different sources
• Binding—Binds approx. 8 mg of GST-tagged fusion protein per mL of resin
Applications:
• Discover a new protein:protein interaction from a cell lysate
• Confirm a putative interaction from a cell lysate or with a previously purified protein
• Extract protein:protein interaction information from in vitro transcription/translation lysates
You provide the tagged fusion protein as the "bait" and the cells expressing the putative protein interaction target ("prey"), and the Pull-Down Kits provide everything else: cell lysis buffer, microcentrifuge spin columns, tag-specific affinity resin (agarose beads) and optimized buffers and protocol. The Pull-Down Kits are designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers.
Related Products
Pierce™ His Protein Interaction Pull-Down Kit
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Assay Type: | Affinity Purification |
|---|---|
| For Use With (Equipment): | Microcentrifuge |
| Product Line: | Pierce™ |
| Product Size: | 25 reactions |
| Target Specificity: | Not Target-Specific |
| Technique: | Pull-Down (Tag-Based) |
Contents & storage
Sufficient For: At least 25 pull-down reactions, each using 25 µL of resin
• Glutathione Agarose, 750 µL
• Pull-Down Lysis Buffer, 250 mL
• Glutathione, 1 g
• TBS Pack (makes 500 mL), 1 pack
• Pierce Spin Columns and Accessories, 27 columns
• Microcentrifuge Collection Tubes, 100 tubes
Upon receipt store Pierce Lysis Buffer at room temperature. Store remaining kit components at 4°C.
• Glutathione Agarose, 750 µL
• Pull-Down Lysis Buffer, 250 mL
• Glutathione, 1 g
• TBS Pack (makes 500 mL), 1 pack
• Pierce Spin Columns and Accessories, 27 columns
• Microcentrifuge Collection Tubes, 100 tubes
Upon receipt store Pierce Lysis Buffer at room temperature. Store remaining kit components at 4°C.
