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Catalog Number | Quantity |
---|---|
21516 | 25 reactions kit |
Thermo Fisher Scientific Pierce GST Tag Protein Interaction Pull-Down Kits contains the necessary components to capture and purify proteins that interact with GST-tagged fusion proteins.
Features of GST Tag Protein Interaction Pull-Down Kit:
Applications:
You provide the tagged fusion protein as the 'bait' and the cells expressing the putative protein interaction target ('prey'), and the Pull-Down Kits provide everything else: cell lysis buffer, microcentrifuge spin columns, tag-specific affinity resin (agarose beads) and optimized buffers and protocol. The Pull-Down Kits are designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers.
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Pierce™ His Protein Interaction Pull-Down Kit (Catalog #21277)
Pull-down is a small-scale affinity purification technique similar to immunoprecipitation (IP), except that the antibody function of is replaced by some other affinity system. In this case, the affinity system is a GST-tagged protein that can be captured by glutathione agarose beads. The fusion-tagged protein acts as the bait to capture a putative binding partner (i.e., the prey). In a typical pull-down assay, the immobilized bait protein is incubated with a cell lysate. After the prescribed washing steps, the interactors are selectively eluted for analysis in-gel or by Western blot.