Thermo Scientific™

Pierce™ GST Protein Interaction Pull-Down Kit

Catalog number: 21516
Thermo Scientific™

Pierce™ GST Protein Interaction Pull-Down Kit

Catalog number: 21516
Catalog Number
21516
Unit Size
25 reactions
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
2151625 reactions
-
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Thermo Fisher Scientific Pierce GST Tag Protein Interaction Pull-Down Kits contains the necessary components to capture and purify proteins that interact with GST-tagged fusion proteins.

Features of GST Tag Protein Interaction Pull-Down Kit:

GST pull-down (Product No. 21516)—gently purifies protein interactors of any GST-tagged fusion protein without denaturing
Complete kits—provide all components and detailed protocol for purifying protein:protein interactions
No special equipment needed—use common laboratory equipment and reagents (e.g., microcentrifuge)
Convenient—microcentrifuge spin columns facilitate simple and efficient manipulation of agarose beads, including simple processing of multiple samples
Flexible—instructions include protocols for use with bait and prey proteins from many different sources
Binding—Binds approx. 8 mg of GST-tagged fusion protein per mL of resin

Applications:
• Discover a new protein:protein interaction from a cell lysate
• Confirm a putative interaction from a cell lysate or with a previously purified protein
• Extract protein:protein interaction information from in vitro transcription/translation lysates

You provide the tagged fusion protein as the 'bait' and the cells expressing the putative protein interaction target ('prey'), and the Pull-Down Kits provide everything else: cell lysis buffer, microcentrifuge spin columns, tag-specific affinity resin (agarose beads) and optimized buffers and protocol. The Pull-Down Kits are designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers.

Related Products
Pierce™ His Protein Interaction Pull-Down Kit
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay
Affinity Purification
For Use With (Equipment)
Microcentrifuge
Product Line
Pierce™
Quantity
1 Kit
For Use With (Application)
Pull-Down (Tag-Based)
Target
Not Target-Specific
Type
Pull-Down Kit
Sufficient For
25 Reactions
Format
Kit

Contents & Storage

Sufficient For: At least 25 pull-down reactions, each using 25 μL of resin
• Glutathione Agarose, 750 μL
• Pull-Down Lysis Buffer, 250 mL
• Glutathione, 1 g
• TBS Pack (makes 500 mL), 1 pack
• Pierce Spin Columns and Accessories, 27 columns
• Microcentrifuge Collection Tubes, 100 tubes

Upon receipt store Pierce Lysis Buffer at room temperature. Store remaining kit components at 4°C.

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