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Thermo Scientific™
Pierce™ GST Protein Interaction Pull-Down Kit
Easily capture and affinity purify binding partners of GST-tagged fusion proteins with these glutathione (GSH) agarose beads and complete reagent set.
| Catalog Number | Quantity |
|---|---|
| 21516 | 25 reactions kit |
Catalog number 21516
Price (USD)
521.65
Online Exclusive
572.00Save 50.35 (9%)
Each
Quantity:
25 reactions kit
Price (USD)
521.65
Online Exclusive
572.00Save 50.35 (9%)
Each
Thermo Fisher Scientific Pierce GST Tag Protein Interaction Pull-Down Kits contains the necessary components to capture and purify proteins that interact with GST-tagged fusion proteins.
Features of GST Tag Protein Interaction Pull-Down Kit:
- GST pull-down (Product No. 21516)—gently purifies protein interactors of any GST-tagged fusion protein without denaturing
- Complete kits—provide all components and detailed protocol for purifying protein:protein interactions
- No special equipment needed—use common laboratory equipment and reagents (e.g., microcentrifuge)
- Convenient—microcentrifuge spin columns facilitate simple and efficient manipulation of agarose beads, including simple processing of multiple samples
- Flexible—instructions include protocols for use with bait and prey proteins from many different sources
- Binding—Binds approx. 8 mg of GST-tagged fusion protein per mL of resin
Applications:
- Discover a new protein:protein interaction from a cell lysate
- Confirm a putative interaction from a cell lysate or with a previously purified protein
- Extract protein:protein interaction information from in vitro transcription/translation lysates
You provide the tagged fusion protein as the 'bait' and the cells expressing the putative protein interaction target ('prey'), and the Pull-Down Kits provide everything else: cell lysis buffer, microcentrifuge spin columns, tag-specific affinity resin (agarose beads) and optimized buffers and protocol. The Pull-Down Kits are designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers.
Related Products
Pierce™ His Protein Interaction Pull-Down Kit (Catalog #21277)
For Research Use Only. Not for use in diagnostic procedures.
Pull-down is a small-scale affinity purification technique similar to immunoprecipitation (IP), except that the antibody function of is replaced by some other affinity system. In this case, the affinity system is a GST-tagged protein that can be captured by glutathione agarose beads. The fusion-tagged protein acts as the bait to capture a putative binding partner (i.e., the prey). In a typical pull-down assay, the immobilized bait protein is incubated with a cell lysate. After the prescribed washing steps, the interactors are selectively eluted for analysis in-gel or by Western blot.
Specifications
AssayAffinity Purification
Bed Volume750 μL
For Use With (Application)Pull-Down (Tag-Based)
For Use With (Equipment)Microcentrifuge
FormatKit
FormulationSlurry: 50 %
Quantity25 reactions kit
Sufficient For25 Reactions
TargetNot Target-Specific
Capacity (Metric)1.5 mL
Product LinePierce™
TypePull-Down Kit
Unit SizeEach
Contents & Storage
Sufficient For: At least 25 pull-down reactions, each using 25 μL of resin
• Glutathione Agarose, 750 μL
• Pull-Down Lysis Buffer, 250 mL
• Glutathione, 1 g
• TBS Pack (makes 500 mL), 1 pack
• Pierce Spin Columns and Accessories, 27 columns
• Microcentrifuge Collection Tubes, 100 tubes
Upon receipt store Pierce Lysis Buffer at room temperature. Store remaining kit components at 4°C.
• Glutathione Agarose, 750 μL
• Pull-Down Lysis Buffer, 250 mL
• Glutathione, 1 g
• TBS Pack (makes 500 mL), 1 pack
• Pierce Spin Columns and Accessories, 27 columns
• Microcentrifuge Collection Tubes, 100 tubes
Upon receipt store Pierce Lysis Buffer at room temperature. Store remaining kit components at 4°C.