CMRL Medium, no glutamine

CMRL Medium was originally developed by Connaught Medical Research Laboratories for the growth of Earle's 'L'cells under serum-free conditions. Gibco™ CMRL Medium is also especially useful for cloning monkey kidney cells and for growth of many other mammalian cell lines when supplemented with horse or calf serum.

This CMRL Medium is modified as follows:

With: Phenol Red

Without: L-glutamine, HEPES

The complete formulation is available.

Gibco™ CMRL Medium is a modified version of Medium 199. CMRL medium is particularly rich in nucleosides and vitamins.

CMRL Medium contains no proteins or growth factors. Therefore, CMRL Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). CMRL Medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.