Thermo Scientific Pierce SMPT is a long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form strong (hindered) yet cleavable (reducible) disulfide bonds with cysteine sulfhydryls.
Features of SMPT:
• Reactive groups: NHS ester and pyridyldithiol • Reactive towards: amino and sulfhydryl groups • Cleavable with disulfide reducing agents • Water-insoluble (dissolve first in DMF or DMSO) • Contains a hindered disulfide bond; has formed immunotoxins with improved stability • In vitro, an SMPT conjugate was as effective as conjugates formed with SPDP and 2-IT • Compare to other varieties of SPDP-type reagents, including pegylated forms
Product References: Crosslinker Application Guide -- search for recent literature references for this product