Thermo Scientific™

BS(PEG)5 (PEGylated bis(sulfosuccinimidyl)suberate)

Catalog number:  21581

 Related applications:

Protein Labeling & Crosslinking

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BS(PEG)5 is a bis-succinimide ester-activated PEG compound used for crosslinking between primary amines (—NH2) in proteins and other molecules. The N-hydroxysuccinimide ester (NHS) groups at either end of the PEG5 spacer react specifically and efficiently with lysine and N-terminal amino groups at pH 7-9 to form stable amide bonds.

Features of BS(PEG)5:

Reactive groups: sulfo-NHS ester (both ends)
Reactive towards: amino groups (primary amines)
• NHS esters react with—NH2 groups at pH 7-9, forming stable amide bonds
• Water-soluble; compare to non-pegylated BS3 (also soluble) and DSS (water-insoluble)
• Membrane-impermeable, allowing for cell surface labeling
• Irreversibly crosslink proteins or peptides by flexible PEG spacer arms
• Polyethylene glycol spacer arms help maintain conjugate solubility
• Pure compound with defined structure and molecular weight, ensuring reproducible protein-modification effects
• PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
• Ideal for small molecule or peptide conjugations

Alternative Names for BS(PEG)n:

• Bis-NHS-dPEG
• BS(PEO)5
• Bis(NHS)PEO5
• dPEG NHS ester
• Bis-succinimide ester PEG 400

Product References
Crosslinker Application Guide -- search for recent literature references for this product
Bioconjugate Techniques, Academic Press (Part No. 20036)

Related Products
BS(PEG)9 (PEGylated bis(sulfosuccinimidyl)suberate)
BS3 (bis(sulfosuccinimidyl)suberate)
For Research Use Only. Not for use in diagnostic procedures.


Crosslinker Type: Homobifunctional
Labeling Method: Chemical Labeling
Product Line: Pierce™
Product Size: 100 mg
Solubility: Water
Spacer: Extra-Long, Pegylated

Contents & storage

Store desiccated at -20°C. Shipped at ambient temperature.


Manuals & protocols