Thermo Scientific™

Pierce™ Biotin-Fluorescein Conjugate

Catalog number:  22030

 Related applications:

Protein Labeling & Crosslinking

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Thermo Scientific Pierce Biotinylated Proteins include biotin-labeled proteins (BSA), enzymes (HRP, AP) and fluorophores (FITC) for use as controls or signal amplification in IHC via avidin-biotin complex (ABC) techniques.

Biotinylated Fluorescein (FITC) cannot be used to polymerize and amplify the signal to the same degree as biotinylated enzymes because each fluorescent molecule is tagged with only one biotin molecule. However, the fluorescent variant of traditional ABC staining is possible with this unique molecule.

Biotinylated FITC—amplify the fluorescein signal of biotinylated primary antibodies by using avidin or streptavidin plus biotinylated fluorescein (B-FITC)

Biotin, also known as vitamin H, is a small molecule (MW 244.3) that is present in tiny amounts in all living cells and is critical for a number of biological processes. The valeric acid side chain of the biotin molecule can be derivatized in order to incorporate various reactive groups that are used to attach biotin to other molecules. In the context of immunohistochemistry (IHC), biotin is conjugated to antibodies or to the enzyme reporters used to detect target antigens.

Related Products
Pierce™ Bovine Serum Albumin, Biotinylated
Pierce™ Horseradish Peroxidase, Biotinylated
Pierce™ Alkaline Phosphatase, Biotinylated
Pierce™ Biotin
For Research Use Only. Not for use in diagnostic procedures.


Excitation⁄Emission (nm): 494⁄518
Label or Dye: FITC (Fluorescein), Fluorescein (FITC)
Product Line: Pierce™
Product Size: 5 mg
Solubility: DMF (Dimethylformamide)

Contents & storage

Store at 4°C, protected from moisture. Shipped at room temperature.


Manuals & protocols