Glasgow's Minimum Essential Medium (GMEM) was originally developed by Ian McPherson and Michael Stoker as a modification of Eagle's medium. It was utilized to study the genetic factors which affected cell competence. Glasgow's MEM was developed for use with kidney cells lines, such as BHK-21.
This MEM is modified as follows:
|• L-glutamine||• Tryptose phosphate broth|
|• Phenol Red||• Sodium Bicarbonate|
The complete formulation
Glasgow's MEM is unique from other media as it contains twice the concentration of amino acids and vitamins than the original Basal Medium Eagle, and is used without serum. Glasgow's MEM was originally formulated with 10% tryptose phosphate broth.cGMP Manufacturing
Gibco™ Glasgow's MEM is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Glasgow's MEM contains no proteins, lipids, or growth factors. Therefore, Glasgow's MEM requires supplementation, commonly with 10% tryptose phosphate broth. Glasgow's MEM uses a sodium bicarbonate buffer system (2.75 g /) and therefore requires a 5-10% CO2
environment to maintain physiological pH.
Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol