EMCH (N-ε-maleimidocaproic acid hydrazide)

Catalog number: 22106

Thermo Scientific™  Related applications: Protein Labeling & Crosslinking

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Description

Thermo Scientific Pierce EMCH is a mid-length, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).

Features of EMCH:

Reactive groups: maleimide and hydrazide
Reactive towards: sulfhydryl groups and carbonyl (aldehyde) groups
• Mid-length (11.8Å), sulfhydryl-to-aldehyde crosslinker with simple spacer arm (noncleavable)
• Maleimide group reacts with sulfhydryl groups to form stable thioether linkages
• Hydrazide group conjugates to oxidized sugars of glycoproteins and carbohydrates
• Use sodium meta-periodate to oxidize glycosylation (e.g., sialic acid) to reactive aldehyde groups
• Use with EDC to conjugate primary amine of hydrazide group to carboxyl groups

3,3'-N-[ε-Maleimidocaproic acid] hydrazide, trifluoroacetic acid salt (EMCH) is a water-soluble, heterobifunctional, membrane permeable crosslinker. It contains a sulfhydryl reactive maleimide group and carbonyl reactive hydrazide group at each end of a 6-carbon spacer arm. Maleimides react with sulfhydryls at pH 6.5-7.5 to form stable thiol ether bonds, along with release of the maleimide leaving group. Proteins with cysteine residues not involved in disulfide bond formation are targets for maleimide reactive groups. Carbonyl groups, whilst not found naturally on proteins can be formed by ring sugar reduction to form aldehydes which react with hydrazides at pH5.5-7.5.

Applications:
• Chemical crosslinking of glycoproteins to sulfhydryl-containing molecules
• Use with EDC for reactivity toward carboxyl groups

Specifications of EMCH:
We manufacture EMCH to the highest specifications to produce the most specific bioconjugates, ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of EMCH is tested to meet the following minimum specifications:

• NMR purity: >90%
• Melting point: 99-110°C
• Identity: IR scan shows only peaks characteristic of the structure and functional groups of EMCH

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
BMPH
MPBH
KMUH
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Spacer: Mid-Length
Solubility: Water
Product Size: 50 mg
Labeling Method: Chemical Labeling
Crosslinker Type: Heterobifunctional

Contents & storage

Store desiccated at 4°C. Shipped at ambient temperature.

Documents

Manuals & protocols