Thermo Scientific™

BMPH (N-β-maleimidopropionic acid hydrazide)

Catalog number:  22297

Related applications:

Protein Labeling & Crosslinking

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Thermo Scientific Pierce BMPH is a short, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).

Features of BMPH:

Reactive groups: maleimide and hydrazide
Reactive towards: sulfhydryl groups and carbonyl (aldehyde) groups
• Short (8.1A), sulfhydryl-to-aldehyde crosslinker with simple spacer arm (noncleavable)
• Maleimide group reacts with sulfhydryl groups to form stable thioether linkages
• Hydrazide group conjugates to oxidized sugars of glycoproteins and carbohydrates
• Use sodium meta-periodate to oxidize glycosylation (e.g., sialic acid) to reactive aldehyde groups
• Use with EDC to conjugate primary amine of hydrazide group to carboxyl groups

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Products
For Research Use Only. Not for use in diagnostic procedures.


Product Line: Pierce™
Crosslinker Type: Heterobifunctional
Form: Solid
Labeling Method: Chemical Labeling
Reactive Moiety: Amine, Hydrazide, Maleimide
Solubility: Water
Spacer: Short
Product Type: Crosslinker
Quantity: 50 mg

Contents & storage

Store desiccated at 4°C. Shipped at ambient temperature.