Thermo Scientific Pierce MPBH is a long, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).
Features of MPBH:
• Reactive groups:maleimide and hydrazide • Reactive towards: sulfhydryl groups and carbonyl (aldehyde) groups • Long (17.9A), sulfhydryl-to-aldehyde crosslinker with an aromatic ring in its spacer arm (noncleavable) • Maleimide group reacts with sulfhydryl groups to form stable thioether linkages • Hydrazide group conjugates to oxidized sugars of glycoproteins and carbohydrates • Use sodium meta-periodate to oxidize glycosylation (e.g., sialic acid) to reactive aldehyde groups • Use with EDC to conjugate primary amine of hydrazide group to carboxyl groups
Product References: Crosslinker Application Guide -- search for recent literature references for this product